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Creators/Authors contains: "Zhang, Lexiang"

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  1. ; ; ; ; ; ; ; (, Chemical Communications)
    null (Ed.)
    Here, we demonstrate use of a Mg 2+ -dependent, site-specific DNA enzyme (DNAzyme) to cleave oligos from polyacrylamide gel beads, which is suitable for use in drop-based assays. We show that cleavage efficiency is improved by use of a tandem-repeat cleavage site. We further demonstrate that DNAzyme-released oligos function as primers in reverse transcription of cell-released mRNA. 
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