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We demonstrate a higher sensitivity detection of proteins in a photonic crystal platform by including a deep subwavelength feature in the unit cell that locally increases the energy density of light. Through both simulations and experiments, the sensing capability of a deep subwavelength-engineered silicon antislot photonic crystal nanobeam (PhCNB) cavity is compared to that of a traditional PhCNB cavity. The redistribution and local enhancement of the energy density by the 50 nm antislot enable stronger light–molecule interaction at the surface of the antislot and lead to a larger resonance shift upon protein binding. This surface-based energy enhancement is confirmed by experiments demonstrating a nearly 50% larger resonance shift upon attachment of streptavidin molecules to biotin-functionalized antislot PhCNB cavities.

 

Between Florida and Cape Hatteras, North Carolina, the Gulf Stream carries warm, salty waters poleward along the continental slope. This strong current abuts the edge of the South Atlantic Bight (SAB) continental shelf and is thought to influence exchange of waters between the open ocean and the shelf. Observations from a pair of instruments deployed for 19 months in the northern SAB are used here to examine the processes by which the Gulf Stream can impact this exchange. The instrument deployed on the SAB shelf edge shows that the time‐averaged along‐slope flow is surface‐intensified with only few flow reversals at low frequencies (>40‐day period). Time‐averaged cross‐slope flow is onto the SAB shelf in a lower layer and off‐shelf above. Consistent with Ekman dynamics, the magnitude of lower‐layer on‐shelf flow is correlated with the along‐slope velocity, which is in turn controlled by the position and/or transport of the Gulf Stream that flows poleward along the SAB continental slope. In the frequency band associated with downstream‐propagating wave‐like meanders of the Gulf Stream jet (2‐15 day period), currents at the shelf‐edge are characterized by surface‐intensified flow in the along‐ and cross‐slope directions. Estimates of maximum upwelling velocities associated with cyclonic frontal eddies between meander crests occasionally reach 100 m/day.

 

The strong sea‐surface temperature (SST) gradient associated with the Gulf Stream (GS) is widely acknowledged to play an important role in shaping mid‐latitude weather and climate. Despite this, an index for the GS SST gradient has not yet been standardized in the literature. This paper introduces a monthly index for the large‐scale SST gradient across the separated GS based on the time‐varying GS position detected from sea‐surface height. Analysis suggests that the variations in the monthly average SST gradient throughout the year result primarily from SST variability to the north of the GS, with little contribution from SST to the south. The index exhibits a weak periodicity at ∼2 years. Sea level pressure and turbulent heat flux patterns suggest that variability in the large‐scale SST gradient is related to atmospheric (rather than oceanic) forcing. Ocean‐to‐atmosphere feedback does not persist throughout the year, but there is some evidence of wintertime feedback.

 

Large-language models (LLMs) such as GPT-4 caught the interest of many scientists. Recent studies suggested that these models could be useful in chemistry and materials science. To explore these possibilities, we organized a hackathon. This article chronicles the projects built as part of this hackathon. Participants employed LLMs for various applications, including predicting properties of molecules and materials, designing novel interfaces for tools, extracting knowledge from unstructured data, and developing new educational applications. The diverse topics and the fact that working prototypes could be generated in less than two days highlight that LLMs will profoundly impact the future of our fields. The rich collection of ideas and projects also indicates that the applications of LLMs are not limited to materials science and chemistry but offer potential benefits to a wide range of scientific disciplines. 

In situ observations from a 19‐month deployment of current‐ and pressure‐sensor equipped inverted echo sounders (CPIESs) along and across the Gulf Stream near Cape Hatteras capture spatial and temporal variability where this western boundary current separates from the continental margin. Regional hydrographic casts and two temperature cross‐sections spanning the Gulf Stream southeast of Cape Hatteras are used with the CPIESs' records of acoustic travel time to infer changes in thermocline depthD_Tand Gulf Stream position. Wave‐like Gulf Stream meanders are observed where the Stream approaches the separation location with periods less than 15 days, wavelengths less than 500‐km, and phase speeds between 40 and 70 km d⁻¹. Though meander amplitudes typically decrease by ∼30% on the final approach to Cape Hatteras, some signals are still coherent across the Gulf Stream separation location. Temporal variability in meander intensity may be related to the Loop Current ∼1,400 km upstream. Mesoscale variability is strongest downstream of the separation location where Gulf Stream position is no longer constrained by the steep continental slope. Low frequency transport changes in the Florida Straits are correlated with sea‐surface height gradients along the entire South Atlantic Bight (SAB) and withD_Tinferred at the CPIES sites. The correlations withD_Tare likely due to coherent transport anomalies in the Gulf Stream approaching the separation location, which then drive Gulf Stream position changes downstream of the separation location. The patterns of coherent transport anomalies may reflect large‐scale atmospheric forcing patterns or rapid equatorward propagation of barotropic signals along the SAB.

 

The Northwest Atlantic, which has exhibited evidence of accelerated warming compared to the global ocean, also experienced several notable marine heatwaves (MHWs) over the last decade. We analyze spatiotemporal patterns of surface and subsurface temperature structure across the Northwest Atlantic continental shelf and slope to assess the influences of atmospheric and oceanic processes on ocean temperatures. Here we focus on MHWs from 2015/16 and examine their physical drivers using observational and reanalysis products. We find that a combination of jet stream latitudinal position and ocean advection, mainly due to warm core rings shed by the Gulf Stream, plays a role in MHW development. While both atmospheric and oceanic drivers can lead to MHWs they have different temperature signatures with each affecting the vertical structure differently and horizontal spatial patterns of a MHW. Northwest Atlantic MHWs have significant socio-economic impacts and affect commercially important species such as squid and lobster.

 

microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 microbial monocultures, users can search known and unknown MS/MS spectra and link them to their respective microbial producers via MS/MS fragmentation patterns. Identification of microbe-derived metabolites and relative producers without a priori knowledge will vastly enhance the understanding of microorganisms’ role in ecology and human health.

 

Few studies have addressed the nutritional ecology of galagos. Observations of galagos in the wild reveal that they rely on fruits and invertebrates to varying degrees depending on their availability. We conducted a 6‐week comparative dietary analysis of a colony of captive‐housed northern greater galagos (Otolemur garnettii), which included five females and six males with known life histories. We compared two experimental diets. The first was fruit dominated and the second was invertebrate dominated. For each diet, we examined dietary intake and apparent dry matter digestibility over the course of 6 weeks. We found significant differences between the apparent digestibility of the diets, with the “invertebrate” diet being more digestible than the “frugivorous” diet. The lower apparent digestibility of the “frugivorous” diet was driven by the higher fiber contents of the fruits provided to the colony. However, variation in apparent digestibility of both diets was found among individual galagos. The experimental design used in this study may provide useful dietary data for the management of captive colonies of galagos and other strepsirrhine primates. This study may also be helpful for understanding the nutritional challenges faced by free‐ranging galagos through time and across geographic space.

 

Purpose To develop a novel model composed solely of Col I and Col III with the lower and upper limits set to include the ratios of Col I and Col III at 3:1 and 9:1 in which the structural and mechanical behavior of the resident CM can be studied. Further, the progression of fibrosis due to change in ratios of Col I:Col III was tested. Methods Collagen gels with varying Col I:Col III ratios to represent a healthy (3:1) and diseased myocardial tissue were prepared by manually casting them in wells. Absorbance assay was performed to confirm the gelation of the gels. Rheometric analysis was performed on each of the collagen gels prepared to determine the varying stiffnesses and rheological parameters of the gels made with varying ratios of Col I:Col III. Second Harmonic Generation (SHG) was performed to observe the 3D characterization of the collagen samples. Scanning Electron microscopy was used for acquiring cross sectional images of the lyophilized collagen gels. AC16 CM (human) cell lines were cultured in the prepared gels to study cell morphology and behavior as a result of the varying collagen ratios. Cellular proliferation was studied by performing a Cell Trace Violet Assay and the applied force on each cell was measured by means of Finite Element Analysis (FEA) on CM from each sample. Results Second harmonic generation microscopy used to image Col I, displayed a decrease in acquired image intensity with an increase in the non-second harmonic Col III in 3:1 gels. SEM showed a fiber-rich structure in the 3:1 gels with well-distributed pores unlike the 9:1 gels or the 1:0 controls. Rheological analysis showed a decrease in substrate stiffness with an increase of Col III, in comparison with other cases. CM cultured within 3:1 gels exhibited an elongated rod-like morphology with an average end-to-end length of 86 ± 28.8 µm characteristic of healthy CM, accompanied by higher cell growth in comparison with other cases. Finite element analysis used to estimate the forces exerted on CM cultured in the 3:1 gels, showed that the forces were well dispersed, and not concentrated within the center of cells, in comparison with other cases. Conclusion This study model can be adopted to simulate various biomechanical environments in which cells crosstalk with the Collagen-matrix in diseased pathologies to generate insights on strategies for prevention of fibrosis. 

We study many‐body localization (MBL) from the perspective of integrals of motion (IOMs). MBL can be understood phenomenologically through the existence of macroscopically many localized IOMs. We develop a systematic procedure based on IOM to calculate many‐body quantities. Displacement transformations made clear that any operator can be expanded in 1‐,2‐ ...n‐particles terms. We use this property to develop a systematic procedure to approximately calculate IOMs and many‐body quantities. We characterize the decay with distance of the IOM's and their interactions through effective localization lengths. For all values of disorder the typical IOMs are localized, suggesting the importance of rare fluctuations in understanding the MBL‐to‐ergodic transition.image