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Impurities immersed into a surrounding ultra-cold Bose gas experience interactions mediated by the surrounding many-body environment. If one focuses on two impurities that are sufficiently close to each other, they can form a bipolaron pair. Here, we discuss how the standard methods based on linearizing the condensate field lead to results only valid in the weak coupling regime and for sufficiently large impurity separations. We show how those shortcomings can be remedied within the Born–Oppenheimer approximation by accounting for boson–boson interactions already on the mean-field level.

 

Abstract DNA replication occurs through an intricately regulated series of molecular events and is fundamental for genome stability 1,2 . At present, it is unknown how the locations of replication origins are determined in the human genome. Here we dissect the role of topologically associating domains (TADs) 3–6 , subTADs 7 and loops 8 in the positioning of replication initiation zones (IZs). We stratify TADs and subTADs by the presence of corner-dots indicative of loops and the orientation of CTCF motifs. We find that high-efficiency, early replicating IZs localize to boundaries between adjacent corner-dot TADs anchored by high-density arrays of divergently and convergently oriented CTCF motifs. By contrast, low-efficiency IZs localize to weaker dotless boundaries. Following ablation of cohesin-mediated loop extrusion during G1, high-efficiency IZs become diffuse and delocalized at boundaries with complex CTCF motif orientations. Moreover, G1 knockdown of the cohesin unloading factor WAPL results in gained long-range loops and narrowed localization of IZs at the same boundaries. Finally, targeted deletion or insertion of specific boundaries causes local replication timing shifts consistent with IZ loss or gain, respectively. Our data support a model in which cohesin-mediated loop extrusion and stalling at a subset of genetically encoded TAD and subTAD boundaries is an essential determinant of the locations of replication origins in human S phase. 

ABSTRACT We present the results of a new, deeper, and complete search for high-redshift 6.5 < z < 9.3 quasars over 977 deg2 of the VISTA Kilo-Degree Infrared Galaxy (VIKING) survey. This exploits a new list-driven data set providing photometry in all bands Z, Y, J, H, Ks, for all sources detected by VIKING in J. We use the Bayesian model comparison (BMC) selection method of Mortlock et al., producing a ranked list of just 21 candidates. The sources ranked 1, 2, 3, and 5 are the four known z > 6.5 quasars in this field. Additional observations of the other 17 candidates, primarily DESI Legacy Survey photometry and ESO FORS2 spectroscopy, confirm that none is a quasar. This is the first complete sample from the VIKING survey, and we provide the computed selection function. We include a detailed comparison of the BMC method against two other selection methods: colour cuts and minimum-χ2 SED fitting. We find that: (i) BMC produces eight times fewer false positives than colour cuts, while also reaching 0.3 mag deeper, (ii) the minimum-χ2 SED-fitting method is extremely efficient but reaches 0.7 mag less deep than the BMC method, and selects only one of the four known quasars. We show that BMC candidates, rejected because their photometric SEDs have high χ2 values, include bright examples of galaxies with very strong [O iii] λλ4959,5007 emission in the Y band, identified in fainter surveys by Matsuoka et al. This is a potential contaminant population in Euclid searches for faint z > 7 quasars, not previously accounted for, and that requires better characterization. 

Protein therapeutics are powerful tools in the fight against diabetes, cancers, growth disorders, and many other debilitating diseases. However, availability is limited due to cost and complications of production from living organisms. To make life‐saving protein therapeutics more available to the world, the possibility of magistral or point‐of‐care protein therapeutic production has gained focus. The recent invention and optimization of lyophilized “cell‐free” protein synthesis reagents and its demonstrated ability to produce highly active versions of FDA‐approved cancer therapeutics have increased its potential for low‐cost, single‐batch, magistral medicine. Here we present for the first time the concept of increased oxygen mass transfer in small‐batch, cell‐free protein synthesis (CFPS) reactions through air‐water foams. These “hydrofoam” reactions increased CFPS yields by up to 100%. Contrary to traditional protein synthesis using living organisms, where foam bubbles cause cell‐lysis and production losses, hydrofoam CFPS reactions are “cell‐free” and better tolerate foaming. Simulation and experimental results suggest that oxygen transfer is limiting in even small volume batch CFPS reactors and that the hydrofoam format improved oxygen transfer. This is further supported by CFPS reactions achieving higher yields when oxygen gas replaces air in the headspace of batch reactions. Improving CFPS yields with hydrofoam reduces the overall cost of biotherapeutic production, increasing availability to the developing world. Beyond protein therapeutic production, hydrofoam CFPS could also be used to enhance other CFPS applications including biosensing, biomanufacturing, and biocatalysis.

 

Acute lymphocytic leukemia (ALL) is a common childhood cancer in the United States, with over 6000 new cases diagnosed each year. Administration of bacterial asparaginase (ASNase) has improved survival rates to nearly 80%, however these therapeutics have high incidence of immunological neutralization and serum activity must be monitored for most effective treatment regimens. Here, a 72% improvement in cell‐free protein synthesis (CFPS) of FDA approvedl‐asparaginase (crisantaspase) is demonstrated by employing an aspartate‐fed‐batch reactor format. A CFPS‐based ASNase activity assay as a tool for therapeutic regimentation and production quality control is also presented. This work suggests that shelf‐stable and low‐costEscherichia coli‐based CFPS reactions may be employed on‐demand to 1) synthesize biologics on‐site for patient administration, 2) verify biologic activity for dosage calculations, and 3) monitor therapeutic activity in human serum during the treatment regimen. The combination of both therapeutic production and activity assessment introduces a concept of synergistic utility for bacterial cell lysates in modern medical treatment. Indeed, recent work with CFPS biosensors supports a not‐too‐distant future when shelf‐stableE. coliCFPS systems are used to diagnose, treat, and monitor treatment of diseases in the clinical setting.

 

Abstract The Review summarizes much of particle physics and cosmology. Using data from previous editions, plus 2,143 new measurements from 709 papers, we list, evaluate, and average measured properties of gauge bosons and the recently discovered Higgs boson, leptons, quarks, mesons, and baryons. We summarize searches for hypothetical particles such as supersymmetric particles, heavy bosons, axions, dark photons, etc. Particle properties and search limits are listed in Summary Tables. We give numerous tables, figures, formulae, and reviews of topics such as Higgs Boson Physics, Supersymmetry, Grand Unified Theories, Neutrino Mixing, Dark Energy, Dark Matter, Cosmology, Particle Detectors, Colliders, Probability and Statistics. Among the 120 reviews are many that are new or heavily revised, including a new review on Machine Learning, and one on Spectroscopy of Light Meson Resonances. The Review is divided into two volumes. Volume 1 includes the Summary Tables and 97 review articles. Volume 2 consists of the Particle Listings and contains also 23 reviews that address specific aspects of the data presented in the Listings. The complete Review (both volumes) is published online on the website of the Particle Data Group (pdg.lbl.gov) and in a journal. Volume 1 is available in print as the PDG Book. A Particle Physics Booklet with the Summary Tables and essential tables, figures, and equations from selected review articles is available in print, as a web version optimized for use on phones, and as an Android app. 

A search for supersymmetry targeting the direct production of winos and higgsinos is conducted in final states with either two leptons (eorμ) with the same electric charge, or three leptons. The analysis uses 139 fb⁻¹ofppcollision data at$$ \sqrt{s} $$$\sqrt{s}$= 13 TeV collected with the ATLAS detector during Run 2 of the Large Hadron Collider. No significant excess over the Standard Model expectation is observed. Simplified and complete models with and withoutR-parity conservation are considered. In topologies with intermediate states including eitherWhorWZpairs, wino masses up to 525 GeV and 250 GeV are excluded, respectively, for a bino of vanishing mass. Higgsino masses smaller than 440 GeV are excluded in a naturalR-parity-violating model with bilinear terms. Upper limits on the production cross section of generic events beyond the Standard Model as low as 40 ab are obtained in signal regions optimised for these models and also for anR-parity-violating scenario with baryon-number-violating higgsino decays into top quarks and jets. The analysis significantly improves sensitivity to supersymmetric models and other processes beyond the Standard Model that may contribute to the considered final states.

 

A search for pair-produced vector-like quarks using events with exactly one lepton (eor$$\mu $$$\mu$), at least four jets including at least oneb-tagged jet, and large missing transverse momentum is presented. Data from proton–proton collisions at a centre-of-mass energy of$$\sqrt{s}=$$$\sqrt{s}=$13 $$\text {TeV}$$$\text{TeV}$, recorded by the ATLAS detector at the LHC from 2015 to 2018 and corresponding to an integrated luminosity of 139 fb$$^{-1}$$${}^{-1}$, are analysed. Vector-like partnersTandBof the top and bottom quarks are considered, as is a vector-likeXwith charge$$+5/3$$$+5/3$, assuming their decay into aW,Z, or Higgs boson and a third-generation quark. No significant deviations from the Standard Model expectation are observed. Upper limits on the production cross-section ofTandBquark pairs as a function of their mass are derived for various decay branching ratio scenarios. The strongest lower limits on the masses are 1.59 $$\text {TeV}$$$\text{TeV}$assuming mass-degenerate vector-like quarks and branching ratios corresponding to the weak-isospin doublet model, and 1.47 $$\text {TeV}$$$\text{TeV}$(1.46 $$\text {TeV}$$$\text{TeV}$) for exclusive$$T \rightarrow Zt$$$T\to Zt$($$B/X \rightarrow Wt$$$B/X\to Wt$) decays. In addition, lower limits on theTandBquark masses are derived for all possible branching ratios.

 

Measurements of Higgs boson production cross-sections are carried out in the diphoton decay channel using 139 fb⁻¹ofppcollision data at$$ \sqrt{s} $$$\sqrt{s}$= 13 TeV collected by the ATLAS experiment at the LHC. The analysis is based on the definition of 101 distinct signal regions using machine-learning techniques. The inclusive Higgs boson signal strength in the diphoton channel is measured to be$$ {1.04}_{-0.09}^{+0.10} $$${1.04}_{-0.09}^{+0.10}$. Cross-sections for gluon-gluon fusion, vector-boson fusion, associated production with aWorZboson, and top associated production processes are reported. An upper limit of 10 times the Standard Model prediction is set for the associated production process of a Higgs boson with a single top quark, which has a unique sensitivity to the sign of the top quark Yukawa coupling. Higgs boson production is further characterized through measurements of Simplified Template Cross-Sections (STXS). In total, cross-sections of 28 STXS regions are measured. The measured STXS cross-sections are compatible with their Standard Model predictions, with ap-value of 93%. The measurements are also used to set constraints on Higgs boson coupling strengths, as well as on new interactions beyond the Standard Model in an effective field theory approach. No significant deviations from the Standard Model predictions are observed in these measurements, which provide significant sensitivity improvements compared to the previous ATLAS results.