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Existing three-dimensional (3D) culture techniques are limited by trade-offs between throughput, capacity for high-resolution imaging in living state, and geometric control. Here, we introduce a modular microscale hanging drop culture where simple design elements allow high replicates for drug screening, direct on-chip real-time or high-resolution confocal microscopy, and geometric control in 3D. Thousands of spheroids can be formed on our microchip in a single step and without any selective pressure from specific matrices. Microchip cultures from human LN229 glioblastoma and patient-derived mouse xenograft cells retained genomic alterations of originating tumors based on mate pair sequencing. We measured response to drugs over time with real-time microscopy on-chip. Last, by engineering droplets to form predetermined geometric shapes, we were able to manipulate the geometry of cultured cell masses. These outcomes can enable broad applications in advancing personalized medicine for cancer and drug discovery, tissue engineering, and stem cell research. 

Aims. HD 206893 is a nearby debris disk star that hosts a previously identified brown dwarf companion with an orbital separation of ∼10 au. Long-term precise radial velocity (RV) monitoring, as well as anomalies in the system proper motion, has suggested the presence of an additional, inner companion in the system. Methods. Using information from ongoing precision RV measurements with the HARPS spectrograph, as well as Gaia host star astrometry, we have undertaken a multi-epoch search for the purported additional planet using the VLTI/GRAVITY instrument. Results. We report a high-significance detection over three epochs of the companion HD 206893c, which shows clear evidence for Keplerian orbital motion. Our astrometry with ∼50−100 μarcsec precision afforded by GRAVITY allows us to derive a dynamical mass of 12.7$ ^{+1.2}_{-1.0} $ M Jup and an orbital separation of 3.53$ ^{+0.08}_{-0.06} $ au for HD 206893c. Our fits to the orbits of both companions in the system use both Gaia astrometry and RVs to also provide a precise dynamical estimate of the previously uncertain mass of the B component, and therefore allow us to derive an age of 155 ± 15 Myr for the system. We find that theoretical atmospheric and evolutionary models that incorporate deuterium burning for HD 206893c, parameterized by cloudy atmosphere models as well as a “hybrid sequence” (encompassing a transition from cloudy to cloud-free), provide a good simultaneous fit to the luminosity of both HD 206893B and c. Thus, accounting for both deuterium burning and clouds is crucial to understanding the luminosity evolution of HD 206893c. Conclusions. In addition to using long-term RV information, this effort is an early example of a direct imaging discovery of a bona fide exoplanet that was guided in part by Gaia astrometry. Utilizing Gaia astrometry is expected to be one of the primary techniques going forward for identifying and characterizing additional directly imaged planets. In addition, HD 206893c is an example of an object narrowly straddling the deuterium-burning limit but unambiguously undergoing deuterium burning. Additional discoveries like this may therefore help clarify the discrimination between a brown dwarf and an extrasolar planet. Lastly, this discovery is another example of the power of optical interferometry to directly detect and characterize extrasolar planets where they form, at ice-line orbital separations of 2−4 au. 

We report the time-resolved spectral analysis of a bright near-infrared and moderate X-ray flare of Sgr A ⋆ . We obtained light curves in the M , K , and H bands in the mid- and near-infrared and in the 2 − 8 keV and 2 − 70 keV bands in the X-ray. The observed spectral slope in the near-infrared band is νL ν  ∝  ν 0.5 ± 0.2 ; the spectral slope observed in the X-ray band is νL ν  ∝  ν −0.7 ± 0.5 . Using a fast numerical implementation of a synchrotron sphere with a constant radius, magnetic field, and electron density (i.e., a one-zone model), we tested various synchrotron and synchrotron self-Compton scenarios. The observed near-infrared brightness and X-ray faintness, together with the observed spectral slopes, pose challenges for all models explored. We rule out a scenario in which the near-infrared emission is synchrotron emission and the X-ray emission is synchrotron self-Compton. Two realizations of the one-zone model can explain the observed flare and its temporal correlation: one-zone model in which the near-infrared and X-ray luminosity are produced by synchrotron self-Compton and a model in which the luminosity stems from a cooled synchrotron spectrum. Both models can describe the mean spectral energy distribution (SED) and temporal evolution similarly well. In order to describe the mean SED, both models require specific values of the maximum Lorentz factor γ max , which differ by roughly two orders of magnitude. The synchrotron self-Compton model suggests that electrons are accelerated to γ max  ∼ 500, while cooled synchrotron model requires acceleration up to γ max  ∼ 5 × 10 4 . The synchrotron self-Compton scenario requires electron densities of 10 10 cm −3 that are much larger than typical ambient densities in the accretion flow. Furthermore, it requires a variation of the particle density that is inconsistent with the average mass-flow rate inferred from polarization measurements and can therefore only be realized in an extraordinary accretion event. In contrast, assuming a source size of 1  R S , the cooled synchrotron scenario can be realized with densities and magnetic fields comparable with the ambient accretion flow. For both models, the temporal evolution is regulated through the maximum acceleration factor γ max , implying that sustained particle acceleration is required to explain at least a part of the temporal evolution of the flare. 

Abstract The Pandora Software Development Kit and algorithm libraries provide pattern-recognition logic essential to the reconstruction of particle interactions in liquid argon time projection chamber detectors. Pandora is the primary event reconstruction software used at ProtoDUNE-SP, a prototype for the Deep Underground Neutrino Experiment far detector. ProtoDUNE-SP, located at CERN, is exposed to a charged-particle test beam. This paper gives an overview of the Pandora reconstruction algorithms and how they have been tailored for use at ProtoDUNE-SP. In complex events with numerous cosmic-ray and beam background particles, the simulated reconstruction and identification efficiency for triggered test-beam particles is above 80% for the majority of particle type and beam momentum combinations. Specifically, simulated 1 GeV/ c charged pions and protons are correctly reconstructed and identified with efficiencies of 86.1 $$\pm 0.6$$ ± 0.6 % and 84.1 $$\pm 0.6$$ ± 0.6 %, respectively. The efficiencies measured for test-beam data are shown to be within 5% of those predicted by the simulation. 

Abstract The rapid development of general-purpose computing on graphics processing units (GPGPU) is allowing the implementation of highly-parallelized Monte Carlo simulation chains for particle physics experiments. This technique is particularly suitable for the simulation of a pixelated charge readout for time projection chambers, given the large number of channels that this technology employs. Here we present the first implementation of a full microphysical simulator of a liquid argon time projection chamber (LArTPC) equipped with light readout and pixelated charge readout, developed for the DUNE Near Detector. The software is implemented with an end-to-end set of GPU-optimized algorithms. The algorithms have been written in Python and translated into CUDA kernels using Numba, a just-in-time compiler for a subset of Python and NumPy instructions. The GPU implementation achieves a speed up of four orders of magnitude compared with the equivalent CPU version. The simulation of the current induced on 10^3 pixels takes around 1 ms on the GPU, compared with approximately 10 s on the CPU. The results of the simulation are compared against data from a pixel-readout LArTPC prototype.