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  1. Free, publicly-accessible full text available January 1, 2025
  2. Free, publicly-accessible full text available October 1, 2024
  3. Mapping molecular deformation and forces in protein biomaterials is critical to understanding mechanochemistry.

     
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    Free, publicly-accessible full text available December 7, 2024
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  6. Since the early 1990s, single-molecule detection in solution at room temperature has enabled direct observation of single biomolecules at work in real time and under physiological conditions, providing insights into complex biological systems that the traditional ensemble methods cannot offer. In particular, recent advances in single-molecule tracking techniques allow researchers to follow individual biomolecules in their native environments for a timescale of seconds to minutes, revealing not only the distinct pathways these biomolecules take for downstream signaling but also their roles in supporting life. In this review, we discuss various single-molecule tracking and imaging techniques developed to date, with an emphasis on advanced three-dimensional (3D) tracking systems that not only achieve ultrahigh spatiotemporal resolution but also provide sufficient working depths suitable for tracking single molecules in 3D tissue models. We then summarize the observables that can be extracted from the trajectory data. Methods to perform single-molecule clustering analysis and future directions are also discussed.

     
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    Free, publicly-accessible full text available June 14, 2024
  7. In this work, a deep learning-based method, STED-flimGANE, is introduced to achieve enhanced STED imaging resolution under a low STED-beam power and photon-starved conditions.

     
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    Free, publicly-accessible full text available June 1, 2024
  8. Periasamy, Ammasi ; So, Peter T. ; König, Karsten (Ed.)
    Free, publicly-accessible full text available April 25, 2024
  9. We demonstrate how addition of polyvinylpyrrolidone (PVP, a non-adsorbing polymer) affects the rheology of concentrated aqueous suspensions of colloidal alumina particles.

     
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  10. Abstract

    DNA has emerged as a powerful substrate for programming information processing machines at the nanoscale. Among the DNA computing primitives used today, DNA strand displacement (DSD) is arguably the most popular, with DSD-based circuit applications ranging from disease diagnostics to molecular artificial neural networks. The outputs of DSD circuits are generally read using fluorescence spectroscopy. However, due to the spectral overlap of typical small-molecule fluorescent reporters, the number of unique outputs that can be detected in parallel is limited, requiring complex optical setups or spatial isolation of reactions to make output bandwidths scalable. Here, we present a multiplexable sequencing-free readout method that enables real-time, kinetic measurement of DSD circuit activity through highly parallel, direct detection of barcoded output strands using nanopore sensor array technology (Oxford Nanopore Technologies’ MinION device). These results increase DSD output bandwidth by an order of magnitude over what is currently feasible with fluorescence spectroscopy.

     
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