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Stimulated Raman projection tomography is a label-free volumetric chemical imaging technology allowing three-dimensional (3D) reconstruction of chemical distribution in a biological sample from the angle-dependent stimulated Raman scattering projection images. However, the projection image acquisition process requires rotating the sample contained in a capillary glass held by a complicated sample rotation stage, limiting the volumetric imaging speed, and inhibiting the study of living samples. Here, we report a tilt-angle stimulated Raman projection tomography (TSPRT) system which acquires angle-dependent projection images by utilizing tilt-angle beams to image the sample from different azimuth angles sequentially. The TSRPT system, which is free of sample rotation, enables rapid scanning of different views by a tailor-designed four-galvo-mirror scanning system. We present the design of the optical system, the theory, and calibration procedure for chemical tomographic reconstruction. 3D vibrational images of polystyrene beads and C. elegans are demonstrated in the C-H vibrational region.

 

preprint, currently in review 

Given a data matrix 𝐷, a submatrix 𝑆 of 𝐷 is an order-preserving submatrix (OPSM) if there is a permutation of the columns of 𝑆, under which the entry values of each row in 𝑆 are strictly increasing. OPSM mining is widely used in real-life applications such as identifying coexpressed genes and finding customers with similar preference. However, noise is ubiquitous in real data matrices due to variable experimental conditions and measurement errors, which makes conventional OPSM mining algorithms inapplicable. No previous work on OPSM has ever considered uncertain value intervals using the well-established possible world semantics. We establish two different definitions of significant OPSMs based on the possible world semantics: (1) expected support-based and (2) probabilistic frequentness-based. An optimized dynamic programming approach is proposed to compute the probability that a row supports a particular column permutation, with a closed-form formula derived to efficiently handle the special case of uniform value distribution and an accurate cubic spline approximation approach that works well with any uncertain value distributions. To efficiently check the probabilistic frequentness, several effective pruning rules are designed to efficiently prune insignificant OPSMs; two approximation techniques based on the Poisson and Gaussian distributions, respectively, are proposed for further speedup. These techniques are integrated into our two OPSM mining algorithms, based on prefix-projection and Apriori, respectively. We further parallelize our prefix-projection-based mining algorithm using PrefixFPM, a recently proposed framework for parallel frequent pattern mining, and we achieve a good speedup with the number of CPU cores. Extensive experiments on real microarray data demonstrate that the OPSMs found by our algorithms have a much higher quality than those found by existing approaches. 

A bidirectional brain interface with both “write” and “read” functions can be an important tool for fundamental studies and potential clinical treatments for neurological diseases. Herein, a miniaturized multifunctional fiber‐based optoacoustic emitter (mFOE) is reported thatintegrates simultaneous optoacoustic stimulation for “write” and electrophysiology recording of neural circuits for “read”. Because of the intrinsic ability of neurons to respond to acoustic wave, there is no requirement of the viral transfection. The orthogonality between optoacoustic waves and electrical field provides a solution to avoid the interference between electrical stimulation and recording. The stimulation function of the mFOE is first validated in cultured ratcortical neurons using calcium imaging. In vivo application of mFOE for successful simultaneous optoacoustic stimulation and electrical recording of brain activities is confirmed in mouse hippocampus in both acute and chronical applications up to 1 month. Minor brain tissue damage is confirmed after these applications. The capability of simultaneous neural stimulation and recording enabled by mFOE opens up new possibilities for the investigation of neural circuits and brings new insights into the study of ultrasound neurostimulation.

 

Understanding metabolic heterogeneity is critical for optimizing microbial production of valuable chemicals, but requires tools that can quantify metabolites at the single‐cell level over time. Here, longitudinal hyperspectral stimulated Raman scattering (SRS) chemical imaging is developed to directly visualize free fatty acids in engineeredEscherichia coliover many cell cycles. Compositional analysis is also developed to estimate the chain length and unsaturation of the fatty acids in living cells. This method reveals substantial heterogeneity in fatty acid production among and within colonies that emerges over the course of many generations. Interestingly, the strains display distinct types of production heterogeneity in an enzyme‐dependent manner. By pairing time‐lapse and SRS imaging, the relationship between growth and production at the single‐cell level are examined. The results demonstrate that cell‐to‐cell production heterogeneity is pervasive and provides a means to link single‐cell and population‐level production.

 

Operable under ambient light and providing chemical selectivity, stimulated Raman scattering (SRS) microscopy opens a new window for imaging molecular events on a human subject, such as filtration of topical drugs through the skin. A typical approach for volumetric SRS imaging is through piezo scanning of an objective lens, which often disturbs the sample and offers a low axial scan rate. To address these challenges, we have developed a deformable mirror-based remote-focusing SRS microscope, which not only enables high-quality volumetric chemical imaging without mechanical scanning of the objective but also corrects the system aberrations simultaneously. Using the remote-focusing SRS microscope, we performed volumetric chemical imaging of living cells and captured in real time the dynamic diffusion of topical chemicals into human sweat pores.