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ABSTRACT The current generation of (sub)mm-telescopes has allowed molecular line emission to become a major tool for studying the physical, kinematic, and chemical properties of extragalactic systems, yet exploiting these observations requires a detailed understanding of where emission lines originate within the Milky Way. In this paper, we present 60 arcsec (∼3 pc) resolution observations of many 3 mm band molecular lines across a large map of the W49 massive star-forming region (∼100 pc × 100 pc at 11 kpc), which were taken as part of the ‘LEGO’ IRAM-30m large project. We find that the spatial extent or brightness of the molecular line transitions are not well correlated with their critical densities, highlighting abundance and optical depth must be considered when estimating line emission characteristics. We explore how the total emission and emission efficiency (i.e. line brightness per H2 column density) of the line emission vary as a function of molecular hydrogen column density and dust temperature. We find that there is not a single region of this parameter space responsible for the brightest and most efficiently emitting gas for all species. For example, we find that the HCN transition shows high emission efficiency at high column density (1022 cm−2) and moderate temperatures (35 K), whilst e.g. N2H+ emits most efficiently towards lower temperatures (1022 cm−2; <20 K). We determine $X_{\mathrm{CO} (1-0)} \sim 0.3 \times 10^{20} \, \mathrm{cm^{-2}\, (K\, km\, s^{-1})^{-1}}$, and $\alpha _{\mathrm{HCN} (1-0)} \sim 30\, \mathrm{M_\odot \, (K\, km\, s^{-1}\, pc^2)^{-1}}$, which both differ significantly from the commonly adopted values. In all, these results suggest caution should be taken when interpreting molecular line emission.