Search for: All records

The versatile functions of fluorescent proteins (FPs) as fluorescence biomarkers depend on their intrinsic chromophores interacting with the protein environment. Besides X-ray crystallography, vibrational spectroscopy represents a highly valuable tool for characterizing the chromophore structure and revealing the roles of chromophore–environment interactions. In this work, we aim to benchmark the ground-state vibrational signatures of a series of FPs with emission colors spanning from green, yellow, orange, to red, as well as the solvated model chromophores for some of these FPs, using wavelength-tunable femtosecond stimulated Raman spectroscopy (FSRS) in conjunction with quantum calculations. We systematically analyzed and discussed four factors underlying the vibrational properties of FP chromophores: sidechain structure, conjugation structure, chromophore conformation, and the protein environment. A prominent bond-stretching mode characteristic of the quinoidal resonance structure is found to be conserved in most FPs and model chromophores investigated, which can be used as a vibrational marker to interpret chromophore–environment interactions and structural effects on the electronic properties of the chromophore. The fundamental insights gained for these light-sensing units (e.g., protein active sites) substantiate the unique and powerful capability of wavelength-tunable FSRS in delineating FP chromophore properties with high sensitivity and resolution in solution and protein matrices. The comprehensive characterization for various FPs across a colorful palette could also serve as a solid foundation for future spectroscopic studies and the rational engineering of FPs with diverse and improved functions. 

The topic of engineering identity is neither new nor complete in its coverage within current literature. In fact, although this body of work predates the last ten years, researchers have argued that some of the most significant burgeoning in this area has occurred in the last decade. By applying both quantitative and qualitative lenses to this inquiry, researchers have concluded that, much like a STEM identity, an engineering identity describes how students see themselves, their competence and potential for success in the academic and career context of the field. To further examine the latter component i.e. potential for academic and career success, we attend to an emerging concept of an entrepreneurial engineering identity. This preliminary work unfolded organically; the authors’ primary goal involved a larger Interpretative Phenomenological Analysis (IPA) study that investigated persistence and advanced degree aspirations among 20 Black male engineering undergraduate students from a variety of institutional settings. While we did not intentionally seek to examine this emerging component of engineering identity, our preliminary analysis of participants’ interview data led us down this path. What we observed was a latent phenomenon of interest among participants: these Black male engineering undergraduates recurringly articulated clear intentions for academic and career opportunities that integrated business components into their engineering realities. Kegan’s (1984, 1994) Theory of Meaning-Making provided a framework for understanding how participants perceived the development of business acumen as a strategy for ascending existing corporate/organizational structures, creating new business pathways, and promoting corporate social responsibility. Based on these findings, the authors were inspired to explore the conceptual development of an entrepreneurial engineering identity and its practical application to engineering degree (re)design, student academic advisory and career planning. 

The topic of engineering identity is neither new nor complete in its coverage within current literature. In fact, although this body of work predates the last ten years, researchers have argued that some of the most significant burgeoning in this area has occurred in the last decade. By applying both quantitative and qualitative lenses to this inquiry, researchers have concluded that, much like a STEM identity, an engineering identity describes how students see themselves, their competence and potential for success in the academic and career context of the field. To further examine the latter component i.e. potential for academic and career success, we attend to an emerging concept of an entrepreneurial engineering identity. This preliminary work unfolded organically; the authors’ primary goal involved a larger Interpretative Phenomenological Analysis (IPA) study that investigated persistence and advanced degree aspirations among 20 Black male engineering undergraduate students from a variety of institutional settings. While we did not intentionally seek to examine this emerging component of engineering identity, our preliminary analysis of participants’ interview data led us down this path. What we observed was a latent phenomenon of interest among participants: these Black male engineering undergraduates recurringly articulated clear intentions for academic and career opportunities that integrated business components into their engineering realities. Kegan’s (1984, 1994) Theory of Meaning-Making provided a framework for understanding how participants perceived the development of business acumen as a strategy for ascending existing corporate/organizational structures, creating new business pathways, and promoting corporate social responsibility. Based on these findings, authors were inspired to explore the conceptual development of an entrepreneurial engineering identity and its practical application to engineering degree (re)design, student academic advisory and career planning.