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  1. ABSTRACT

    A lactose‐containing monomer,N‐(2‐lactosylethyl)acrylamide, was synthesized and polymerized withN‐hydroxyethyl acrylamide and 1 wt % ofN,N'‐methylenebis(acrylamide) and potassium persulfate as the initiator to produce hydrogels. The weight percent ofN‐(2‐lactosylethyl)acrylamide were increased from 0 to 100% in increments of 10%. Hydrogels were successfully produced with up to 90 wt % ofN‐(2‐lactosylethyl)acrylamide. Gelation was confirmed by inverted vial tests and rheology measurements. The as‐prepared hydrogels were used for papain stabilization against heat burden and papain that was loaded into hydrogels showed 45% more activity after heating as compared to papain that was heated without hydrogel stabilization. This hydrogel stabilization technique has potential applications in preserving enzyme activity. © 2016 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem.2016,54, 2507–2514

     
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