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Trapping of strain in layers deposited during extrusion‐based (fused filament fabrication) 3D printing has previously been documented. If fiber‐level strain trapping can be understood sufficiently and controlled, 3D shape‐memory polymer parts could be simultaneously fabricated and programmed via printing (programming via printing; PvP), thereby achieving precisely controlled 3D‐to‐3D transformations of complex part geometries. Yet, because previous studies have only examined strain trapping in solid printed parts—such as layers or 3D objects with 100% infill—fundamental aspects of the PvP process and the potential for PvP to be applied to printing of porous 3D parts remain poorly understood. This work examines the extent to which strain can be trapped in individual fibers and in fibers that span negative space and the extent to which infill geometry affects the magnitude and recovery of strain trapped in porous PvP‐fabricated 3D parts. Additionally, multiaxial shape change of porous PvP‐fabricated 3D parts are for the first time studied, modeled, and applied in a proof‐of‐concept application. This work demonstrates the feasibility of strain trapping in individual fibers in 1D, 2D, and 3D PvP‐fabricated parts and illustrates the potential for PvP to provide new strategies to address unmet needs in biomedical and other fields.

 

Combination of stem cell technology and 3D biofabrication approaches provides physiological similarity to in vivo tissues and the capability of repairing and regenerating damaged human tissues. Mesenchymal stem cells (MSCs) have been widely used for regenerative medicine applications because of their immunosuppressive properties and multipotent potentials. To obtain large amount of high-quality MSCs without patient donation and invasive procedures, we differentiated MSCs from human-induced pluripotent stem cells (hiPSC-MSCs) using serum-free E6 media supplemented with only one growth factor (bFGF) and two small molecules (SB431542 and CHIR99021). The differentiated cells showed a high expression of common MSC-specific surface markers (CD90, CD73, CD105, CD106, CD146, and CD166) and a high potency for osteogenic and chondrogenic differentiation. With these cells, we have been able to manufacture MSC tissue rings with high consistency and robustness in pluronic-coated reusable PDMS devices. The MSC tissue rings were characterized based on inner diameter and outer ring diameter and observed cell-type-dependent tissue contraction induced by cell-matrix interaction. Our approach of simplified hiPSC-MSC differentiation, modular fabrication procedure, and serum-free culture conditions has a great potential for scalable manufacturing of MSC tissue rings for different regenerative medicine applications. 

Fabrication of multiscale, multimaterial 3D structures at high resolution is difficult using current technologies. This is especially significant when working with mechanically weak hydrogels. Here, a new hybrid laser printing (HLP) technology is reported to print complex, multiscale, multimaterial, 3D hydrogel structures with microscale resolution. This technique utilizes sequential additive and subtractive modes of fabrication, that are typically considered as mutually exclusive due to differences in their material processing conditions. Further, compared to current laser writing systems that enforce stringent processing depth limits, HLP is shown to fabricate structures at any depth inside the material. As a proof‐of‐principle, a Mayan pyramid with embedded cube frame is printed using synthetic polyethylene glycol diacrylate (PEGDA) hydrogel. Printing of ready‐to‐use open‐well chips with embedded microchannels is also demonstrated using PEGDA and gelatin methacrylate (GelMA) hydrogels for potential applications in biomedical sciences. Next, HLP is used in additive–additive modes to print multiscale 3D structures spanning in size from centimeter to micrometers within minutes, which is followed by printing of 3D, multimaterial, multiscale structures using this technology. Overall, this work demonstrates that HLP's fabrication versatility can potentially offer a unique opportunity for a range of applications in optics and photonics, biomedical sciences, microfluidics, etc.