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Abstract Separation of microparticles and cells serves a critical step in many applications such as in biological analyses, food production, chemical processing, and medical diagnostics. Sorting on the microscale exhibits certain advantages in comparison with that on the macroscale as it requires minuscule sample or reagents volume and thus reduced analysis cycle time, smaller size of devices, and lower fabrication costs. Progresses have been made over time to improve the efficiency of these microscale particle manipulation techniques. Many different techniques have been used to attain accurate particle sorting and separation in a continuous manner on the microscale level, which can be categorized as either passive or active methods. Passive techniques achieve accurate manipulation of particles through their interaction with surrounding flow by carefully designed channel structures, without using external fields. As an alternative, active techniques utilize external fields (e.g., acoustic, electronic, optical, and magnetic field, etc.) to realize desired pattern of motion for particles with specific properties. Among numerous active methods for microfluidic particle sorting, the magnetic field has been widely used in biomedical and chemical applications to achieve mixing, focusing, and separating of reagents and bioparticles. This paper aims to provide a thorough review on the classic and most up-to-date magnetic sorting and separation techniques to manipulate microparticles including the discussions on the basic concept, working principle, experimental details, and device performance. 

Abstract Separation of microparticles and cells serves a critical step in many applications such as in biological analyses, food production, chemical processing, and medical diagnostics. Sorting on the microscale exhibits certain advantages in comparison with that on the macroscale as it requires minuscule sample or reagents volume and thus reduced analysis cycle time, smaller size of devices, and lower fabrication costs. Progresses have been made over time to improve the efficiency of these microscale particle manipulation techniques. Many different techniques have been used to attain accurate particle sorting and separation in a continuous manner on the microscale level, which can be categorized as either passive or active methods. Passive techniques achieve accurate manipulation of particles through their interaction with surrounding flow by carefully designed channel structures, without using external fields. As an alternative, active techniques utilize external fields (e.g., acoustic, electronic, optical, and magnetic field, etc.) to realize desired pattern of motion for particles with specific properties. Among numerous active methods for microfluidic particle sorting, the magnetic field has been widely used in biomedical and chemical applications to achieve mixing, focusing, and separating of reagents and bioparticles. This paper aims to provide a thorough review on the classic and most up-to-date magnetic sorting and separation techniques to manipulate microparticles including the discussions on the basic concept, working principle, experimental details, and device performance. 

The phenomenon of ferrofluid-water mixing is investigated using a double-layer magnetic micromixer, in which a layer of micromagnet bars is placed immediately below the fluid layer. A wavy pattern of the ferrofluid–water interface is surprisingly observed at each micromagnet responsible for improved mixing. The mechanism causing the wavy mixing is discovered and analyzed through experimental measurements and numerical simulations, and the mixing efficiency under different flow conditions is discussed. For flows with Re ≪ 1, the resultant steep gradient of opposing magnetic forces by micromagnets in the ferrofluid region gives rise to a local pressure source that induces a transverse/spanwise pressure gradient and activates momentum transfer between fluids. The current finding enables effective localized mixing of ferrofluids with a small footprint and, thus, has great potential to achieve fast mixing for high-throughput flows with an integrated parallel system of multiple microfluidic channels and micromagnets. 

As the focus for CRISPR/Cas-edited plants moves from proof-of-concept to real-world applications, precise gene manipulation will increasingly require concurrent multiplex editing for polygenic traits. A common approach for editing across multiple sites is to design one guide RNA (gRNA) per target; however, this complicates construct assembly and increases the possibility of off-target mutations. In this study, we utilized one gRNA to target MYB186, a known positive trichome regulator, as well as its paralogs MYB138 and MYB38 at a consensus site for mutagenesis in hybrid poplar (Populus tremula × P. alba INRA 717-1B4). Unexpected duplications of MYB186 and MYB138 resulted in eight alleles for the three targeted genes in the hybrid poplar. Deep sequencing and polymerase chain reaction analyses confirmed editing across all eight targets in nearly all of the resultant glabrous mutants, ranging from small indels to large genomic dropouts, with no off-target activity detected at four potential sites. This highlights the effectiveness of a single gRNA targeting conserved exonic regions for multiplex editing. Additionally, cuticular wax and whole-leaf analyses showed a complete absence of triterpenes in the trichomeless mutants, hinting at a previously undescribed role for the nonglandular trichomes of poplar.