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Extensive research has been conducted to resolve small-scale microlayer and bubble nucleation and departure processes in flow boiling, building on controlled pool boiling studies. Large-scale two-phase flow structures, such as Taylor bubbles, are known to locally modify transport due to their wakes and varying surrounding liquid film thickness. However, the effect of interaction of such large-scale flow processes with bubble nucleation is not yet well characterized. Wakes may drive premature nucleating bubble departure, or conversely, suppress boiling due to boundary layer quenching, significantly affecting overall heat transfer. To explore such phenomena, a two-phase flow boiling visualization facility is developed to collect simultaneous high-speed visualization and infrared (IR) thermal imaging temperature distribution data. The test cell channel is 420 mm long with a 10 mm × 10 mm internal square-cross section. A transparent conductive indium tin oxide (ITO) coated sapphire window serves as a heater and IR interface for measuring the internal wall temperature. The facility is charged with a low boiling point fluid (HFE7000) to reduce uncertainties from heat loss to the laboratory environment. Vertical saturated flow boiling wake-nucleation interaction experiments are performed for varying liquid volume flow rates (0.5 − 1.5 L min-1, laminar-to-turbulent Re) and heat fluxes (0 − 100 kW m-2). Discrete vapor slugs are injected to explore interactions with nucleate boiling processes. By measuring film heater power, surface temperature distributions, and pressures, local instantaneous heat transfer coefficients (HTC) can be obtained. Results will be applied to assess simulations at matched conditions for void fraction, and size statistics of flow structures.