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Scientific instruments have long been a vital part of science, paving pathways to remarkable scientific advancements. Such advancements have changed the world both socially and culturally, especially in the past few decades. Students can be introduced to this idea through the concepts of nature of science (NOS): scientific observations are often filtered through apparatus, inferences can be made through observations, and science is a socially and culturally embedded practice. The curriculum often fails to emphasize the role of instruments in scientific practices, even in teaching laboratories. This study uses semistructured interviews to investigate the cognitive (thoughts) and affective (feelings) domains of first-year university students as they relate to scientific instrumentation, including students’ ideas of instruments. First, the study probed how general chemistry students conceptualize scientific instruments in relation to the three NOS notions. Second, students’ practices related to experimental data evaluation were investigated as data collection is a large part of psychomotor learning in laboratory. Third, students’ affective states toward learning about instruments were queried. The interview results suggested that a majority of participants acknowledge some ideas of NOS, while a few students displayed an advanced understanding when discussing scientific instruments and also tended to have higher interest and motivation toward learning about instruments.