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An Optimized Whole‐Mount Immunofluorescence Method for Shoot Apices

https://doi.org/10.1002/cpz1.101

Tran, Thu M. ; Demesa‐Arevalo, Edgar ; Kitagawa, Munenori ; Garcia‐Aguilar, Marcelina ; Grimanelli, Daniel ; Jackson, David ( April 2021 , Current Protocols)

Abstract
The localization of a protein provides important information about its biological functions. The visualization of proteins by immunofluorescence has become an essential approach in cell biology. Here, we describe an easy‐to‐follow immunofluorescence protocol to localize proteins in whole‐mount tissues of maize (Zea mays) and Arabidopsis. We present the whole‐mount immunofluorescence procedure using maize ear primordia and Arabidopsis inflorescence apices as examples, followed by tips and suggestions for each step. In addition, we provide a supporting protocol to describe the use of an ImageJ plug‐in to analyze colocalization. This protocol has been optimized to observe proteins in 2‐5 mm maize ear primordia or in developing Arabidopsis inflorescence apices; however, it can be used as a reference to perform whole‐mount immunofluorescence in other plant tissues and species. © 2021 Wiley Periodicals LLC.

Basic Protocol: Whole‐mount immunofluorescence for maize and Arabidopsis shoot apices

Support Protocol: Measure colocalization by JACoP plugin in ImageJ

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Gene duplication at the Fascicled ear1 locus controls the fate of inflorescence meristem cells in maize.

Du, Yanfang ; Lunde, China ; Li, Yunfu ; Jackson, David ; Hake, Sarah ; Zhang, Zuxin. ( February 2021 , Proceedings of the National Academy of Sciences of the United States of America)
null (Ed.)
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Single-cell RNA sequencing of developing maize ears facilitates functional analysis and trait candidate gene discovery

https://doi.org/10.1016/j.devcel.2020.12.015

Xu, Xiaosa ; Crow, Megan ; Rice, Brian R. ; Li, Forrest ; Harris, Benjamin ; Liu, Lei ; Demesa-Arevalo, Edgar ; Lu, Zefu ; Wang, Liya ; Fox, Nathan ; et al ( February 2021 , Developmental Cell)
null (Ed.)
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High-Throughput CRISPR/Cas9 Mutagenesis Streamlines Trait Gene Identification in Maize

https://doi.org/10.1105/tpc.19.00934

Liu, Hai-Jun ; Jian, Liumei ; Xu, Jieting ; Zhang, Qinghua ; Zhang, Maolin ; Jin, Minliang ; Peng, Yong ; Yan, Jiali ; Han, Baozhu ; Liu, Jie ; et al ( May 2020 , The Plant Cell)

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Control of meristem determinacy by trehalose 6-phosphate phosphatases is uncoupled from enzymatic activity

https://doi.org/10.1038/s41477-019-0394-z

Claeys, Hannes ; Vi, Son Lang ; Xu, Xiaosa ; Satoh-Nagasawa, Namiko ; Eveland, Andrea L. ; Goldshmidt, Alexander ; Feil, Regina ; Beggs, Grace A. ; Sakai, Hajime ; Brennan, Richard G. ; et al ( April 2019 , Nature Plants)

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