NSF PAR Search | NSF Public Access Repository

Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher. Some full text articles may not yet be available without a charge during the embargo (administrative interval).
What is a DOI Number?

Some links on this page may take you to non-federal websites. Their policies may differ from this site.

Implications of a multiscale structure of the yeast nuclear pore complex

https://doi.org/10.1016/j.molcel.2023.08.025

Akey, Christopher W.; Echeverria, Ignacia; Ouch, Christna; Nudelman, Ilona; Shi, Yi; Wang, Junjie; Chait, Brian T.; Sali, Andrej; Fernandez-Martinez, Javier; Rout, Michael P. (September 2023, Molecular Cell)

Full Text Available
Comprehensive structure and functional adaptations of the yeast nuclear pore complex

https://doi.org/10.1016/j.cell.2021.12.015

Akey, Christopher W.; Singh, Digvijay; Ouch, Christna; Echeverria, Ignacia; Nudelman, Ilona; Varberg, Joseph M.; Yu, Zulin; Fang, Fei; Shi, Yi; Wang, Junjie; et al (January 2022, Cell)

Full Text Available
Replication and single-cycle delivery of SARS-CoV-2 replicons

https://doi.org/10.1126/science.abj8430

Ricardo-Lax, Inna; Luna, Joseph M.; Thao, Tran Thi; Le Pen, Jérémie; Yu, Yingpu; Hoffmann, H.-Heinrich; Schneider, William M.; Razooky, Brandon S.; Fernandez-Martinez, Javier; Schmidt, Fabian; et al (November 2021, Science)

Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
more » « less
Full Text Available
One Ring to Rule them All? Structural and Functional Diversity in the Nuclear Pore Complex

https://doi.org/10.1016/j.tibs.2021.01.003

Fernandez-Martinez, Javier; Rout, Michael P. (July 2021, Trends in Biochemical Sciences)

Full Text Available

Search for: All records