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Award ID contains: 1916691

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  1. Abstract Unraveling the complexity of biological systems relies on the development of new approaches for spatially resolved proteoform‐specific analysis of the proteome. Herein, we employ nanospray desorption electrospray ionization mass spectrometry imaging (nano‐DESI MSI) for the proteoform‐selective imaging of biological tissues. Nano‐DESI generates multiply charged protein ions, which is advantageous for their structural characterization using tandem mass spectrometry (MS/MS) directly on the tissue. Proof‐of‐concept experiments demonstrate that nano‐DESI MSI combined with on‐tissue top‐down proteomics is ideally suited for the proteoform‐selective imaging of tissue sections. Using rat brain tissue as a model system, we provide the first evidence of differential proteoform expression in different regions of the brain. 
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  2. Free, publicly-accessible full text available May 22, 2026
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  5. This study presents an application of TPD-DART-HRMS for plastic, enabling rapid characterization of waste through Kendrick grouping and Tanimoto comparison. 
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    Free, publicly-accessible full text available January 22, 2026