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We show that there exist infinitely many closed 3–manifolds that do not embed in closed symplectic 4–manifolds, disproving a conjecture of Etnyre–Min–Mukherjee. To do this, we construct L–spaces that cannot bound positive- or negative-definite manifolds. The arguments use Heegaard Floer correction terms and instanton moduli spaces. 

Kronheimer and Mrowka asked whether the difference between the four-dimensional clasp number and the slice genus can be arbitrarily large. This question is answered affirmatively by studying a knot invariant derived from equivariant singular instanton theory, and which is closely related to the Chern-Simons functional. This also answers a conjecture of Livingston about slicing numbers. Also studied is the singular instanton Frøyshov invariant of a knot. If defined with integer coefficients, this gives a lower bound for the unoriented slice genus, and is computed for quasi-alternating and torus knots. In contrast, for certain other coefficient rings, the invariant is identified with a multiple of the knot signature. This result is used to address a conjecture by Poudel and Saveliev about traceless SU(2) representations of torus knots. Further, for a concordance between knots with non-zero signature, it is shown that there is a traceless representation of the concordance complement which restricts to non-trivial representations of the knot groups. Finally, some evidence towards an extension of the slice-ribbon conjecture to torus knots is provided.