- Home
- Search Results
- Page 1 of 1
Search for: All records
-
Total Resources3
- Resource Type
-
00030
- Availability
-
30
- Author / Contributor
- Filter by Author / Creator
-
-
Bashir, Rashid (3)
-
Ganguli, Anurup (3)
-
Valera, Enrique (3)
-
Berger, Jacob (2)
-
Kumar, James (2)
-
Lim, Jongwon (2)
-
Mostafa, Ariana (2)
-
White, Karen (2)
-
Aamir, Muhammad (1)
-
Aedma, Surya (1)
-
Araud, Elbashir (1)
-
Aydin, Mehmet Y. (1)
-
Baek, Janice (1)
-
Baltaji, Ali (1)
-
Cunningham, Brian T. (1)
-
Jankelow, Aaron (1)
-
Johnson, Mark (1)
-
Kindratenko, Victoria (1)
-
King, William P. (1)
-
Mady, Mohamed (1)
-
- Filter by Editor
-
-
null (1)
-
& Spizer, S. M. (0)
-
& . Spizer, S. (0)
-
& Ahn, J. (0)
-
& Bateiha, S. (0)
-
& Bosch, N. (0)
-
& Brennan K. (0)
-
& Brennan, K. (0)
-
& Chen, B. (0)
-
& Chen, Bodong (0)
-
& Drown, S. (0)
-
& Ferretti, F. (0)
-
& Higgins, A. (0)
-
& J. Peters (0)
-
& Kali, Y. (0)
-
& Ruiz-Arias, P.M. (0)
-
& S. Spitzer (0)
-
& Spitzer, S. (0)
-
& Spitzer, S.M. (0)
-
(submitted - in Review for IEEE ICASSP-2024) (0)
-
-
Have feedback or suggestions for a way to improve these results?
!
Note: When clicking on a Digital Object Identifier (DOI) number, you will be taken to an external site maintained by the publisher.
Some full text articles may not yet be available without a charge during the embargo (administrative interval).
What is a DOI Number?
Some links on this page may take you to non-federal websites. Their policies may differ from this site.
-
Valera, Enrique ; Jankelow, Aaron ; Lim, Jongwon ; Kindratenko, Victoria ; Ganguli, Anurup ; White, Karen ; Kumar, James ; Bashir, Rashid ( , ACS Nano)
-
Ganguli, Anurup ; Mostafa, Ariana ; Berger, Jacob ; Aydin, Mehmet Y. ; Sun, Fu ; Ramirez, Sarah A. ; Valera, Enrique ; Cunningham, Brian T. ; King, William P. ; Bashir, Rashid ( , Proceedings of the National Academy of Sciences)null (Ed.)The COVID-19 pandemic provides an urgent example where a gap exists between availability of state-of-the-art diagnostics and current needs. As assay protocols and primer sequences become widely known, many laboratories perform diagnostic tests using methods such as RT-PCR or reverse transcription loop mediated isothermal amplification (RT-LAMP). Here, we report an RT-LAMP isothermal assay for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus and demonstrate the assay on clinical samples using a simple and accessible point-of-care (POC) instrument. We characterized the assay by dipping swabs into synthetic nasal fluid spiked with the virus, moving the swab to viral transport medium (VTM), and sampling a volume of the VTM to perform the RT-LAMP assay without an RNA extraction kit. The assay has a limit of detection (LOD) of 50 RNA copies per μL in the VTM solution within 30 min. We further demonstrate our assay by detecting SARS-CoV-2 viruses from 20 clinical samples. Finally, we demonstrate a portable and real-time POC device to detect SARS-CoV-2 from VTM samples using an additively manufactured three-dimensional cartridge and a smartphone-based reader. The POC system was tested using 10 clinical samples, and was able to detect SARS-CoV-2 from these clinical samples by distinguishing positive samples from negative samples after 30 min. The POC tests are in complete agreement with RT-PCR controls. This work demonstrates an alternative pathway for SARS-CoV-2 diagnostics that does not require conventional laboratory infrastructure, in settings where diagnosis is required at the point of sample collection.more » « less