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Abstract Chitin is an abundant, carbon-rich polymer in the marine environment. Chitinase activity has been detected in spent media ofSynechococcusWH7803 cultures—yet it was unclear which specific enzymes were involved. Here we delivered a CRISPR tool into the cells via electroporation to generate loss-of-function mutants of putative candidates and identified ChiA as the enzyme required for the activity detected in the wild type.more » « less
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