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Abstract The advancement of additive manufacturing has significantly transformed the production process of metal components. However, the unique challenges associated with layer-by-layer manufacturing result in anisotropy in the microstructure and uneven mechanical properties of additive-manufactured metal products. Traditional testing methods often fall short of providing the precise mechanical performance evaluations required to meet industry standards. This paper introduces an innovative approach that combines a nondestructive Lamb wave sensing system with a wavenumber analysis method to characterize the mechanical properties of 3D-printed metal panels in multiple directions. Our method employs piezoelectric actuators (PZT) to generate Lamb waves and utilizes a laser Doppler vibrometer (LDV) for non-contact, two-dimensional grid acquisition of the wavefield. The anisotropic properties of the metal 3D-printed structure will be captured in the wavefield, offering an informative dataset for wavenumber analysis. The proposed analytical method includes multi-directional frequency wavenumber analysis and a least-squares-based dispersion curves regression. The integration of the above advanced analytical tools allows for the accurate characterization of the shear wave velocity and Poisson’s ratio of the plate structure. This precise characterization is crucial for ensuring the structural integrity and consistent mechanical properties of 3D-printed metal components. We validated our method using a 3D-printed stainless-steel plate, demonstrating its capability to effectively characterize the multi-directional mechanical properties of additively manufactured metal plates. We expect that our method can provide a nondestructive, time-efficient, and comprehensive quality control solution for additive manufacturing across various industries. 

Abstract Thermoset materials have begun to be applied in additive composite manufacturing due to their ability to withstand high temperatures without losing structural integrity. Meanwhile, the characterization of mechanical properties for additively manufactured composites is critical for ensuring material reliability and safety. However, traditional testing methods struggle to accurately and nondestructively characterize additively manufactured composites due to challenges posed by curing processes, microstructural variability, anisotropic properties of thermoset composites, and the risk of damaging these materials during evaluation. For characterizing the mechanical properties of additive-manufactured thermoset composites, this paper presents a novel method that combines a nondestructive PZT-LDV guided wave sensing system and a wavenumber analysis that fuses multidimensional Fourier transform with dispersion curve regression. For proof of concept, we performed an experiment using our method to measure a 3D-printed thermoset composite panel. Based on our nondestructive approach, two material properties (shear wave velocity and Poisson’s ratio) in multiple directions were successfully determined for the tested panel. We expect this research to introduce a non-contact and efficient method for characterizing various composites and monitoring their property changes after additive manufacturing. 

Abstract Cell patterning techniques play a pivotal role in the development of three-dimensional (3D) engineered tissues, holding significant promise in regenerative medicine, drug screening, and disease research. Current techniques encompass various mechanisms, such as nanoscale topographic patterning, mechanical loading, chemical coating, 3D inkjet printing, electromagnetic fields, and acoustic waves. In this study, we introduce a unique standing bulk waves-based acoustic cell patterning device designed for constructing anisotropic-engineered glioma tissues containing acoustically patterned human glioblastoma cell U251. Our device features two orthogonal pairs of piezoelectric transducers securely mounted on a customized holder. The energy of standing bulk waves generated from these transducers can be transmitted into the medium in a Petri dish through its bottom wall. Cells in the medium can be directed to the local minima of Gor’kov potential fields and trapped by the resultant acoustic radiation force. Through proof-of-concept experiments, we validate the functionality of our acoustic patterning device and assess the morphology and differentiation of U251 cells within the engineered glioma tissues. Our findings reveal that cells can be arranged in different distributions, such as parallel-line-like and lattice-like patterns. Moreover, the aligned cells exhibit more obvious elongation along the cell alignment orientation compared to the result of a control group. We anticipate that this study will catalyze the advancement in contactless cell patterning technologies within tissue engineering, facilitating the development of engineered tissues for applications in regenerative medicine and disease research. 

Abstract Surface acoustic waves (SAWs) have shown great potential for developing sensors for structural health monitoring (SHM) and lab‐on‐a‐chip (LOC) applications. Existing SAW sensors mainly rely on measuring the frequency shifts of high‐frequency (e.g., >0.1 GHz) resonance peaks. This study presents frequency‐locked wireless multifunctional SAW sensors that enable multiple wireless sensing functions, including strain sensing, temperature measurement, water presence detection, and vibration sensing. These sensors leverage SAW resonators on piezoelectric chips, inductive coupling‐based wireless power transmission, and, particularly, a frequency‐locked wireless sensing mechanism that works at low frequencies (e.g., <0.1 GHz). This mechanism locks the input frequency on the slope of a sensor's reflection spectrum and monitors the reflection signal's amplitude change induced by the changes of sensing parameters. The proof‐of‐concept experiments show that these wireless sensors can operate in a low‐power active mode for on‐demand wireless strain measurement, temperature sensing, and water presence detection. Moreover, these sensors can operate in a power‐free passive mode for vibration sensing, with results that agree well with laser vibrometer measurements. It is anticipated that the designs and mechanisms of the frequency‐locked wireless SAW sensors will inspire researchers to develop future wireless multifunctional sensors for SHM and LOC applications. 

Abstract Acoustofluidics has shown great potential in enabling on‐chip technologies for driving liquid flows and manipulating particles and cells for engineering, chemical, and biomedical applications. To introduce on‐demand liquid sample processing and micro/nano‐object manipulation functions to wearable and embeddable electronics, wireless acoustofluidic chips are highly desired. This paper presents wireless acoustofluidic chips to generate acoustic waves carrying sufficient energy and achieve key acoustofluidic functions, including arranging particles and cells, generating fluid streaming, and enriching in‐droplet particles. To enable these functions, the wireless acoustofluidic chips leverage mechanisms, including inductive coupling‐based wireless power transfer (WPT), frequency multiplexing‐based control of multiple acoustic waves, and the resultant acoustic radiation and drag forces. For validation, the wirelessly generated acoustic waves are measured using laser vibrometry when different materials (e.g., bone, tissue, and hand) are inserted between the WPT transmitter and receiver. Moreover, the wireless acoustofluidic chips successfully arrange nanoparticles into different patterns, align cells into parallel pearl chains, generate streaming, and enrich in‐droplet microparticles. This research is anticipated to facilitate the development of embeddable wireless on‐chip flow generators, wearable sensors with liquid sample processing functions, and implantable devices with flow generation and acoustic stimulation abilities for engineering, veterinary, and biomedical applications. 

Abstract Tweezers based on optical, electric, magnetic, and acoustic fields have shown great potential for contactless object manipulation. However, current tweezers designed for manipulating millimeter‐sized objects such as droplets, particles, and small animals exhibit limitations in translation resolution, range, and path complexity. Here, a novel acoustic vortex tweezers system is introduced, which leverages a unique airborne acoustic vortex end effector integrated with a three‐degree‐of‐freedom (DoF) linear motion stage, for enabling contactless, multi‐mode, programmable manipulation of millimeter‐sized objects. The acoustic vortex end effector utilizes a cascaded circular acoustic array, which is portable and battery‐powered, to generate an acoustic vortex with a ring‐shaped energy pattern. The vortex applies acoustic radiation forces to trap and spin an object at its center, simultaneously protecting this object by repelling other materials away with its high‐energy ring. Moreover, The vortex tweezers system facilitates contactless, multi‐mode, programmable object surfing, as demonstrated in experiments involving trapping, repelling, and spinning particles, translating particles along complex paths, guiding particles around barriers, translating and rotating droplets containing zebrafish larvae, and merging droplets. With these capabilities, It is anticipated that the tweezers system will become a valuable tool for the automated, contactless handling of droplets, particles, and bio‐samples in biomedical and biochemical research. 

The graphic illustrates how an external acoustic field stabilizes the SEI layer by enhancing lithium-ion mass transfer at slip lines and kinks, reducing pit formation and promoting a more uniform SEI, ultimately improving battery performance. 

Robotic manipulation of small objects has shown great potential for engineering, biology, and chemistry research. However, existing robotic platforms have difficulty in achieving contactless, high-resolution, 4-degrees-of-freedom (4-DOF) manipulation of small objects, and noninvasive maneuvering of objects in regions shielded by tissue and bone barriers. Here, we present chirality-tunable acoustic vortex tweezers that can tune acoustic vortex chirality, transmit through biological barriers, trap single micro- to millimeter-sized objects, and control object rotation. Assisted by programmable robots, our acoustic systems further enable contactless, high-resolution translation of single objects. Our systems were demonstrated by tuning acoustic vortex chirality, controlling object rotation, and translating objects along arbitrary-shaped paths. Moreover, we used our systems to trap single objects in regions with tissue and skull barriers and translate an object inside a Y-shaped channel of a thick biomimetic phantom. In addition, we showed the function of ultrasound imaging–assisted acoustic manipulation by monitoring acoustic object manipulation via live ultrasound imaging.