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2. PEGylated Dmoc phosphoramidites for sensitive oligodeoxynucleotide synthesis

   https://doi.org/10.1039/D3OB01495A  

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   Using PEGylated Dmoc (pDmoc) phosphoramidites for oligodeoxynucleotide (ODN) synthesis increases the solubility of ODN on solid support and enables the synthesis of longer sensitive ODNs. 

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3. Quantifying Bound Proteins on Pegylated Gold Nanoparticles Using Infrared Spectroscopy

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   Handali, Paul R; Webb, Lauren J (February 2024, ACS Applied Bio Materials)

   Protein−nanoparticle (NP) complexes are nanomaterials that have numerous potential uses ranging from biosensing to biomedical applications such as drug delivery and nanomedicine. Despite their extensive use quantifying the number of bound proteins per NP remains a challenging characterization step that is crucial for further developments of the conjugate, particularly for metal NPs that often interfere with standard protein quantification techniques. In this work, we present a method for quantifying the number of proteins bound to pegylated thiol-capped gold nanoparticles (AuNPs) using an infrared (IR) spectrometer, a readily available instrument. This method takes advantage of the strong IR bands present in proteins and the capping ligands to quantify protein−NP ratios and circumvents the need to degrade the NPs prior to analysis. We show that this method is generalizable where calibration curves made using inexpensive and commercially available proteins such as bovine serum albumin (BSA) can be used to quantify protein−NP ratios for proteins of different sizes and structures. 

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5. Recent Advances in Supramolecular Affinity Separations: Affinity Chromatography and Related Methods

   https://doi.org/10.1201/9781003223405-1  

   Woolfork, A.G.; Iftekhar, S.; Ovbude, S.; Suh, K.; Sharmeen, S.; Kyei, I.; Jones, J.; Hage, D.S. (January 2021, Advances in chromatography)

   Grinberg, Nelu; Carr, Peter W. (Ed.)

   Affinity chromatography is a technique that uses a stationary phase based on the supramolecular interactions that occur in biological systems or mimics of these systems. This method has long been a popular tool for the isolation, measurement, and characterization of specific targets in complex samples. This review discusses the basic concepts of this method and examines recent developments in affinity chromatography and related supramolecular separation methods. Topics that are examined include advances that have occurred in the types of supports, approaches to immobilization, and binding agents that are employed in this method. New developments in the applications of affinity chromatography are also summarized, including an overview on the use of this method for biochemical purification, sample preparation or analysis, chiral separations, and biointeraction studies. 

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