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1. Single-molecule imaging of telomerase reverse transcriptase in human telomerase holoenzyme and minimal RNP complexes

   https://doi.org/10.7554/eLife.08363  

   Wu, Robert Alexander ; Dagdas, Yavuz S ; Yilmaz, S Tunc ; Yildiz, Ahmet ; Collins, Kathleen ( October 2015 , eLife)

   Telomerase synthesizes chromosome-capping telomeric repeats using an active site in telomerase reverse transcriptase (TERT) and an integral RNA subunit template. The fundamental question of whether human telomerase catalytic activity requires cooperation across two TERT subunits remains under debate. In this study, we describe new approaches of subunit labeling for single-molecule imaging, applied to determine the TERT content of complexes assembled in cells or cell extract. Surprisingly, telomerase reconstitutions yielded heterogeneous DNA-bound TERT monomer and dimer complexes in relative amounts that varied with assembly and purification method. Among the complexes, cellular holoenzyme and minimal recombinant enzyme monomeric for TERT had catalytic activity. Dimerization was suppressed by removing a TERT domain linker with atypical sequence bias, which did not inhibit cellular or minimal enzyme assembly or activity. Overall, this work defines human telomerase DNA binding and synthesis properties at single-molecule level and establishes conserved telomerase subunit architecture from single-celled organisms to humans.
2. Single-molecule imaging reveals the translocation and DNA looping dynamics of hepatitis C virus NS3 helicase: Translocation Dynamics of NS3 Helicase

   https://doi.org/10.1002/pro.3136  

   Lin, Chang-Ting ; Tritschler, Felix ; Lee, Kyung Suk ; Gu, Meigang ; Rice, Charles M. ; Ha, Taekjip ( July 2017 , Protein Science)
3. Translocation of cell-penetrating peptides into Candida fungal pathogens: CPP Translocation in Candida

   https://doi.org/10.1002/pro.3203  

   Gong, Zifan ; Karlsson, Amy J. ( September 2017 , Protein Science)
4. Evolution of plant telomerase RNAs: farther to the past, deeper to the roots

   https://doi.org/10.1093/nar/gkab545  

   Fajkus, Petr ; Kilar, Agata ; Nelson, Andrew D ; Holá, Marcela ; Peška, Vratislav ; Goffová, Ivana ; Fojtová, Miloslava ; Zachová, Dagmar ; Fulnečková, Jana ; Fajkus, Jiří ( June 2021 , Nucleic Acids Research)

   Abstract The enormous sequence heterogeneity of telomerase RNA (TR) subunits has thus far complicated their characterization in a wider phylogenetic range. Our recent finding that land plant TRs are, similarly to known ciliate TRs, transcribed by RNA polymerase III and under the control of the type-3 promoter, allowed us to design a novel strategy to characterize TRs in early diverging Viridiplantae taxa, as well as in ciliates and other Diaphoretickes lineages. Starting with the characterization of the upstream sequence element of the type 3 promoter that is conserved in a number of small nuclear RNAs, and the expected minimum TR template region as search features, we identified candidate TRs in selected Diaphoretickes genomes. Homologous TRs were then used to build covariance models to identify TRs in more distant species. Transcripts of the identified TRs were confirmed by transcriptomic data, RT-PCR and Northern hybridization. A templating role for one of our candidates was validated in Physcomitrium patens. Analysis of secondary structure demonstrated a deep conservation of motifs (pseudoknot and template boundary element) observed in all published TRs. These results elucidate the evolution of the earliest eukaryotic TRs, linking the common origin of TRs across Diaphoretickes, and underlying evolutionary transitions in telomeremore »repeats.« less
5. Ancestral Mutation in Telomerase Causes Defects in Repeat Addition Processivity and Manifests As Familial Pulmonary Fibrosis

   https://doi.org/10.1371/journal.pgen.1001352  

   Alder, Jonathan K. ; Cogan, Joy D. ; Brown, Andrew F. ; Anderson, Collin J. ; Lawson, William E. ; Lansdorp, Peter M. ; Phillips, John A. ; Loyd, James E. ; Chen, Julian J.-L. ; Armanios, Mary ; et al ( March 2011 , PLoS Genetics)