Chemiluminescence imaging of bioanalytes using spiroadamantane 1,2‐dioxetanes has gained significant attention due to improved signal‐to‐noise ratios and imaging depth compared to excitation‐based probes, as well as their modifiable scaffolds that offer analyte‐specific responses and tunable emissive properties. Among several strategies employed to amplify signals under aqueous conditions and to shift the emission into the bio‐relevant red region, energy transfer to an adjacent fluorophore is a popular and effective method. This Minireview highlights spiroadamantane 1,2‐dioxetane‐based probes that operate via an energy transfer mechanism to detect bioanalytes both in vitro and in vivo. Probes that display both non‐covalent and covalent interactions with fluorophores, as well as their applications in imaging specific analytes will be discussed.
Ratiometric sensors generally couple binding events or chemical reactions at a distal site to changes in the fluorescence of a core fluorophore scaffold. However, such approaches are often hindered by spectral overlap of the product and reactant species. We provide a strategy to design ratiometric sensors that display dramatic spectral shifts by leveraging the chemoselective reactivity of novel functional groups inserted within fluorophore scaffolds. As a proof‐of‐principle, fluorophores containing a borinate (
- NSF-PAR ID:
- 10035244
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Angewandte Chemie
- Volume:
- 129
- Issue:
- 15
- ISSN:
- 0044-8249
- Page Range / eLocation ID:
- p. 4261-4264
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Abstract Chemiluminescence imaging of bioanalytes using spiroadamantane 1,2‐dioxetanes has gained significant attention due to improved signal‐to‐noise ratios and imaging depth compared to excitation‐based probes, as well as their modifiable scaffolds that offer analyte‐specific responses and tunable emissive properties. Among several strategies employed to amplify signals under aqueous conditions and to shift the emission into the bio‐relevant red region, energy transfer to an adjacent fluorophore is a popular and effective method. This Minireview highlights spiroadamantane 1,2‐dioxetane‐based probes that operate via an energy transfer mechanism to detect bioanalytes both in vitro and in vivo. Probes that display both non‐covalent and covalent interactions with fluorophores, as well as their applications in imaging specific analytes will be discussed.
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