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1. Aseismic Deformation During the 2014 M w 5.2 Karonga Earthquake, Malawi, From Satellite Interferometry and Earthquake Source Mechanisms

   https://doi.org/10.1029/2020GL090930  

   Zheng, Whyjay ; Oliva, Sarah Jaye ; Ebinger, Cynthia ; Pritchard, Matthew E. ( November 2020 , Geophysical Research Letters)

   Aseismic deformation has been suggested as a mechanism to release the accumulated strain in rifts. However, the fraction and the spatial distribution of the aseismic strain are poorly constrained during amagmatic episodes. Using Sentinel‐1 interferograms, we identify the surface deformation associated with the 2014M_w5.2 Karonga earthquake, Malawi, and perform inversions for fault geometry. We also analyze aftershocks and find a variety of source mechanisms within short timescales. A significant discrepancy in the earthquake depth determined by geodesy (3–6 km) and seismology (11–13 km) exists, although both methods indicateM_w5.2. We propose that the surface deformation is caused by aseismic slip from a shallow depth. This vertical partitioning from seismic to aseismic strain is accommodated by intersecting dilatational faults in the shallow upper crust and sedimentary basin, highlighting the importance of considering aseismic deformation in active tectonics and time‐averaged strain patterns, even in rifts with little volcanism.

    

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2. Developing immortal cell lines from Xenopus embryos , four novel cell lines derived from Xenopus tropicalis

   https://doi.org/10.1098/rsob.220089  

   Gorbsky, Gary J. ; Daum, John R. ; Sapkota, Hem ; Summala, Katja ; Yoshida, Hitoshi ; Georgescu, Constantin ; Wren, Jonathan D. ; Peshkin, Leonid ; Horb, Marko E. ( July 2022 , Open Biology)

   The diploid anuran Xenopus tropicalis has emerged as a key research model in cell and developmental biology. To enhance the usefulness of this species, we developed methods for generating immortal cell lines from Nigerian strain (NXR_1018, RRID:SCR_013731) X. tropicalis embryos. We generated 14 cell lines that were propagated for several months. We selected four morphologically distinct lines, XTN-6, XTN-8, XTN-10 and XTN-12 for further characterization. Karyotype analysis revealed that three of the lines, XTN-8, XTN-10 and XTN-12 were primarily diploid. XTN-6 cultures showed a consistent mixed population of diploid cells, cells with chromosome 8 trisomy, and cells containing a tetraploid content of chromosomes. The lines were propagated using conventional culture methods as adherent cultures at 30°C in a simple, diluted L-15 medium containing fetal bovine serum without use of a high CO 2 incubator. Transcriptome analysis indicated that the four lines were distinct lineages. These methods will be useful in the generation of cell lines from normal and mutant strains of X. tropicalis as well as other species of Xenopus . 

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3. Mixotrophic Iron-Oxidizing Thiomonas Isolates from an Acid Mine Drainage-Affected Creek

   https://doi.org/10.1128/AEM.01424-20  

   Akob, Denise M. ; Hallenbeck, Michelle ; Beulig, Felix ; Fabisch, Maria ; Küsel, Kirsten ; Keffer, Jessica L. ; Woyke, Tanja ; Shapiro, Nicole ; Lapidus, Alla ; Klenk, Hans-Peter ; et al ( November 2020 , Applied and Environmental Microbiology)

   Liu, Shuang-Jiang (Ed.)

   ABSTRACT Natural attenuation of heavy metals occurs via coupled microbial iron cycling and metal precipitation in creeks impacted by acid mine drainage (AMD). Here, we describe the isolation, characterization, and genomic sequencing of two iron-oxidizing bacteria (FeOB) species: Thiomonas ferrovorans FB-6 and Thiomonas metallidurans FB-Cd, isolated from slightly acidic (pH 6.3), Fe-rich, AMD-impacted creek sediments. These strains precipitated amorphous iron oxides, lepidocrocite, goethite, and magnetite or maghemite and grew at a pH optimum of 5.5. While Thiomonas spp. are known as mixotrophic sulfur oxidizers and As oxidizers, the FB strains oxidized Fe, which suggests they can efficiently remove Fe and other metals via coprecipitation. Previous evidence for Thiomonas sp. Fe oxidation is largely ambiguous, possibly because of difficulty demonstrating Fe oxidation in heterotrophic/mixotrophic organisms. Therefore, we also conducted a genomic analysis to identify genetic mechanisms of Fe oxidation, other metal transformations, and additional adaptations, comparing the two FB strain genomes with 12 other Thiomonas genomes. The FB strains fall within a relatively novel group of Thiomonas strains that includes another strain (b6) with solid evidence of Fe oxidation. Most Thiomonas isolates, including the FB strains, have the putative iron oxidation gene cyc2 , but only the two FB strains possess the putative Fe oxidase genes mtoAB . The two FB strain genomes contain the highest numbers of strain-specific gene clusters, greatly increasing the known Thiomonas genetic potential. Our results revealed that the FB strains are two distinct novel species of Thiomonas with the genetic potential for bioremediation of AMD via iron oxidation. IMPORTANCE As AMD moves through the environment, it impacts aquatic ecosystems, but at the same time, these ecosystems can naturally attenuate contaminated waters via acid neutralization and catalyzing metal precipitation. This is the case in the former Ronneburg uranium-mining district, where AMD impacts creek sediments. We isolated and characterized two iron-oxidizing Thiomonas species that are mildly acidophilic to neutrophilic and that have two genetic pathways for iron oxidation. These Thiomonas species are well positioned to naturally attenuate AMD as it discharges across the landscape. 

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4. Lithospheric S Wave Velocity Variations Beneath the Mackenzie Mountains and Northern Canadian Cordillera

   https://doi.org/10.1029/2022JB025517  

   Schutt, Derek L. ; Porritt, Robert W. ; Estève, Clément ; Audet, Pascal ; Gosselin, Jeremy M. ; Schaeffer, Andrew J. ; Aster, Richard C. ; Freymueller, Jeffrey T. ; Cubley, Joel F. ( December 2022 , Journal of Geophysical Research: Solid Earth)

   The Mackenzie Mountains (MMs) in the Yukon and Northwest Territories, Canada, are an enigmatic mountain range. They are currently uplifting (Leonard et al., 2008,https//doi.org/10.1029/2007JB005456), yet are about 700 km from the nearest plate boundary. Their arcuate shape is distinct and extends over 100 km eastward from the general trend of the Northern Canadian Cordillera. To better assess the cause and conditions of the current uplift, we processed ambient seismic noise data from a linear array of broadband seismographs crossing the mountains, along with other regional seismic stations, to estimate Rayleigh wave phase velocities between 6 and 40 s periods. From this, we estimated phase velocity dispersion and performed a tomographic inversion to estimateV_S. Tomography reveals a low‐velocity structure that extends upward from the base of the ∼50–66 km thick lithosphere to the upper crust, and we hypothesize that inferred low density and low rigidity associated with theV_Sanomaly localizes the ongoing uplift and thrust‐dominated seismicity of the MMs. Additionally, we find relatively low crustal velocities that extend to the west of the MMs, suggesting that strain transfer from the Gulf of Alaska plate boundary plays a driving role as the crust translates to the northeast and buckles up against the craton consistent with the orogenic float hypothesis of Mazzotti and Hyndman (2002,https//doi.org/10.1130/0091-7613(2002)030〈0495:YCASTA〉2.0.CO;2). Finally, we observe lithospheric azimuthal anisotropy with an NW‐SE fast direction. This is nearly orthogonal to teleseismic shear wave splitting measurements in the central MMs, and suggests that asthenosphere flow and lithospheric strain are not aligned in this region.

    

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5. Isolation, growth, and nitrogen fixation rates of the Hemiaulus-Richelia (diatom-cyanobacterium) symbiosis in culture

   https://doi.org/10.7717/peerj.10115  

   Pyle, Amy E. ; Johnson, Allison M. ; Villareal, Tracy A. ( January 2020 , PeerJ)

   Nitrogen fixers (diazotrophs) are often an important nitrogen source to phytoplankton nutrient budgets in N-limited marine environments. Diazotrophic symbioses between cyanobacteria and diatoms can dominate nitrogen-fixation regionally, particularly in major river plumes and in open ocean mesoscale blooms. This study reports the successful isolation and growth in monocultures of multiple strains of a diatom-cyanobacteria symbiosis from the Gulf of Mexico using a modified artificial seawater medium. We document the influence of light and nutrients on nitrogen fixation and growth rates of the host diatom Hemiaulus hauckii Grunow together with its diazotrophic endosymbiont Richelia intracellularis Schmidt, as well as less complete results on the Hemiaulus membranaceus - R. intracellularis symbiosis. The symbioses rates reported here are for the joint diatom-cyanobacteria unit. Symbiont diazotrophy was sufficient to support both the host diatom and cyanobacteria symbionts, and the entire symbiosis replicated and grew without added nitrogen. Maximum growth rates of multiple strains of H. hauckii symbioses in N-free medium with N 2 as the sole N source were 0.74–0.93 div d −1 . Growth rates followed light saturation kinetics in H. hauckii symbioses with a growth compensation light intensity (E C ) of 7–16 µmol m −2 s −1 and saturation light level (E K ) of 84–110 µmol m −2 s −1 . Nitrogen fixation rates by the symbiont while within the host followed a diel pattern where rates increased from near-zero in the scotophase to a maximum 4–6 h into the photophase. At the onset of the scotophase, nitrogen-fixation rates declined over several hours to near-zero values. Nitrogen fixation also exhibited light saturation kinetics. Maximum N 2 fixation rates (84 fmol N 2 heterocyst −1 h −1 ) in low light adapted cultures (50 µmol m −2 s − 1) were approximately 40–50% of rates (144–154 fmol N 2 heterocyst −1 h −1 ) in high light (150 and 200 µmol m −2 s −1 ) adapted cultures. Maximum laboratory N 2 fixation rates were ~6 to 8-fold higher than literature-derived field rates of the H. hauckii symbiosis. In contrast to published results on the Rhizosolenia-Richelia symbiosis, the H. hauckii symbiosis did not use nitrate when added, although ammonium was consumed by the H. hauckii symbiosis. Symbiont-free host cell cultures could not be established; however, a symbiont-free H. hauckii strain was isolated directly from the field and grown on a nitrate-based medium that would not support DDA growth. Our observations together with literature reports raise the possibility that the asymbiotic H. hauckii are lines distinct from an obligately symbiotic H. hauckii line. While brief descriptions of successful culture isolation have been published, this report provides the first detailed description of the approaches, handling, and methodologies used for successful culture of this marine symbiosis. These techniques should permit a more widespread laboratory availability of these important marine symbioses. 

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