Diazotrophic <i>Trichodesmium</i> impact on UV–Vis radiance and pigment composition in the western tropical South Pacific

Abstract. We assessed the influence of the marine diazotrophic cyanobacterium Trichodesmium on the bio-optical properties of western tropical South Pacific (WTSP) waters (18–22°S, 160°E–160°W) during the February–March 2015 OUTPACE cruise. We performed measurements of backscattering and absorption coefficients, irradiance, and radiance in the euphotic zone with a Satlantic MicroPro free-fall profiler and took Underwater Vision Profiler 5 (UPV5) pictures for counting the largest Trichodesmium spp. colonies. Pigment concentrations were determined by fluorimetry and high-performance liquid chromatography and picoplankton abundance by flow cytometry. Trichome concentration was estimated from pigment algorithms and validated by surface visual counts. The abundance of large colonies counted by the UVP5 (maximum 7093coloniesm−3) was well correlated to the trichome concentrations (maximum 2093trichomesL−1) with an aggregation factor of 600. In the Melanesian archipelago, a maximum of 4715trichomesL−1 was enumerated in pump samples (3.2m) at 20°S,16730°E. High Trichodesmium abundance was always associated with absorption peaks of mycosporine-like amino acids (330, 360nm) and high particulate backscattering, but not with high Chl a fluorescence or blue particulate absorption (440nm). Along the west-to-east transect, Trichodesmium together with Prochlorococcus represented the major part of total chlorophyll concentration; the more »

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Award ID(s):
Publication Date:
NSF-PAR ID:
10077684
Journal Name:
Biogeosciences
Volume:
15
Issue:
16
Page Range or eLocation-ID:
5249 to 5269
ISSN:
1726-4189
4. Abstract. Photoacoustic spectroscopy (PAS) has become a popular technique for measuringabsorption of light by atmospheric aerosols in both the laboratory andfield campaigns. It has low detection limits, measures suspended aerosols,and is insensitive to scattering. But PAS requires rigorous calibration to beapplied quantitatively. Often, a PAS instrument is either filled with a gasof known concentration and absorption cross section, such that the absorptionin the cell can be calculated from the product of the two, or the absorptionis measured independently with a technique such as cavity ring-downspectroscopy. Then, the PAS signal can be regressed upon the known absorptionto determine a calibration slope that reflects the sensitivity constant ofthe cell and microphone. Ozone has been used for calibrating PAS instrumentsdue to its well-known UV–visible absorption spectrum and the ease with whichit can be generated. However, it is known to photodissociate up toapproximately 1120nm via the O3 + $h\mathit{\nu }\phantom{\rule{0.25em}{0ex}}\left(>\mathrm{1.1}\mathrm{eV}\right)\to {\mathrm{O}}_{\mathrm{2}}{\left(}^{\mathrm{3}}{\mathrm{\Sigma }}_{g}^{-}\right)$ + O(3P) pathway, which is likely tolead to inaccuracies in aerosol measurements. Two recent studies haveinvestigated the use of O3 for PASmore »