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Abstract Thionitrous acid (HSNO), the smallest S‐nitrosothiol, is emerging as a potential key intermediate in cellular redox regulation linking two signaling molecules H2S and NO. However, the chemical biology of HSNO remains poorly understood. A major hurdle is the lack of methods for selective detection of HSNO in biological systems. Herein, we report the rational design, synthesis, and evaluation of the first fluorescent probe TAP‐1 for HSNO detection. TAP‐1 showed high selectivity and sensitivity to HSNO in aqueous media and cells, providing a useful tool for understanding the functions of HSNO in biology.
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Highlights in Chemical Glycobiology
Abstract The advances of chemical biology have provided means for a better understanding of complex biological systems at the molecular level as well as solutions to the problems associated with the complex systems. The interdisciplinary approach of chemical biology was also embraced by the research in glycoscience as illustrated in this essay with some of the relevant examples from our group.
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- Award ID(s):
- 1664283
- PAR ID:
- 10080549
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Israel Journal of Chemistry
- Volume:
- 59
- Issue:
- 1-2
- ISSN:
- 0021-2148
- Page Range / eLocation ID:
- p. 18-22
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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