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   https://doi.org/10.1091/mbc.E22-03-0091  

   Navarro, Alexandra P.; Cheeseman, Iain M. (October 2022, Molecular Biology of the Cell)

   Munson, Mary (Ed.)

   Prior work has identified signal sequences and motifs that are necessary and sufficient to target proteins to specific subcellular regions and organelles such as the plasma membrane, nucleus, endoplasmic reticulum, and mitochondria. In contrast, minimal sequence motifs that are sufficient for Golgi localization remain largely elusive. In this work, we identified a 37–amino acid alternative open reading frame (altORF) within the mRNA of the centromere protein CENP-R. This altORF peptide localizes specifically to the cytoplasmic surface of the Golgi apparatus. Through mutational analysis, we identify a minimal 10–amino acid sequence and a critical cysteine residue that are necessary and sufficient for Golgi localization. Pharmacological perturbations suggest that this peptide undergoes lipid modification to promote its localization. Together, our work defines a minimal sequence that is sufficient for Golgi targeting and provide a valuable Golgi marker for live cell imaging. 

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2. Post-Golgi Trafficking and Transport of Cell Wall Components

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5. Spontaneous cortical dynamics from the first years to the golden years

   https://doi.org/10.1073/pnas.2212776120  

   Rempe, Maggie P; Ott, Lauren R; Picci, Giorgia; Penhale, Samantha H; Christopher-Hayes, Nicholas J; Lew, Brandon J; Petro, Nathan M; Embury, Christine M; Schantell, Mikki; Johnson, Hallie J; et al (January 2023, Proceedings of the National Academy of Sciences)

   In the largest and most expansive lifespan magnetoencephalography (MEG) study to date (n = 434, 6 to 84 y), we provide critical data on the normative trajectory of resting-state spontaneous activity and its temporal dynamics. We perform cutting-edge analyses to examine age and sex effects on whole-brain, spatially-resolved relative and absolute power maps, and find significant age effects in all spectral bands in both types of maps. Specifically, lower frequencies showed a negative correlation with age, while higher frequencies positively correlated with age. These correlations were further probed with hierarchical regressions, which revealed significant nonlinear trajectories in key brain regions. Sex effects were found in absolute but not relative power maps, highlighting key differences between outcome indices that are generally used interchangeably. Our rigorous and innovative approach provides multispectral maps indicating the unique trajectory of spontaneous neural activity across the lifespan, and illuminates key methodological considerations with the widely used relative/absolute power maps of spontaneous cortical dynamics. 

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