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Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity microprojectiles (biolistic, or microparticle bombardment) has been an invaluable tool for both agricultural and fundamental plant research. Here, we review the technical aspects and the major applications of the biolistic method for studies involving transient gene expression in plant cells. These studies cover multiple areas of plant research, including gene expression, protein subcellular localization and cell-to-cell movement, plant virology, silencing, and the more recently developed targeted genome editing via transient expression of customized endonucleases.
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Biolistic approach for transient gene expression studies in plants
Since its inception in the late 1980s, the delivery of exogenous nucleic acids into living cells via high-velocity micro-projectiles (biolistic, or micro-particle bombardment) has been an invaluable tool for both agricultural and fundamental plant research. Here, we review the technical aspects and the major applications of the biolistic method for studies involving transient gene expression in plant cells. These studies cover multiple areas of plant research, including gene expression, protein subcellular localization and cell-to-cell movement, plant virology, silencing and the more recently developed targeted genome editing via transient expression of customized endonuclease.
more »
« less
- PAR ID:
- 10092955
- Date Published:
- Journal Name:
- Methods in molecular biology
- ISSN:
- 0097-0816
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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