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1. The Effect of Drought on Transcriptome and Hormonal Profiles in Barley Genotypes With Contrasting Drought Tolerance

   https://doi.org/10.3389/fpls.2020.618491  

   Harb, Amal; Simpson, Craig; Guo, Wenbin; Govindan, Ganesan; Kakani, Vijaya Gopal; Sunkar, Ramanjulu (December 2020, Frontiers in Plant Science)

   null (Ed.)

   Like many cereal crops, barley is also negatively affected by drought stress. However, due to its simple genome as well as enhanced stress resilient nature compared to rice and wheat, barley has been considered as a model to decipher drought tolerance in cereals. In the present study, transcriptomic and hormonal profiles along with several biochemical features were compared between drought-tolerant (Otis) and drought-sensitive (Baronesse) barley genotypes subjected to drought to identify molecular and biochemical differences between the genotypes. The drought-induced decrease in the leaf relative water content, net photosynthesis, and biomass accumulation was relatively low in Otis compared to Baronesse. The hormonal profiles did not reveal significant differences for majority of the compounds other than the GA20 and the cis-zeatin-o-glucoside (c-ZOG), whose levels were greatly increased in Otis compared to Baronesse under drought. The major differences that emerged from the transcriptome analysis are; (1), the overall number of differentially expressed genes was relatively low in drought-tolerant Otis compared to drought-sensitive Baronesse; (2), a wax biosynthesis gene (CER1), and NAC transcription factors were specifically induced in Otis but not in Baronesse; (3), the degree of upregulation of betaine aldehyde dehydrogenase and a homeobox transcription factor (genes with proven roles in imparting drought tolerance), was greater in Otis compared to Baronesse; (4) the extent of downregulation of gene expression profiles for proteins of the reaction center photosystem II (PSII) (D1 and D2) was low in Otis compared to Baronesse; and, (5), alternative splicing (AS) was also found to differ between the genotypes under drought. Taken together, the overall transcriptional responses were low in drought-tolerant Otis but the genes that could confer drought tolerance were either specifically induced or greatly upregulated in the tolerant genotype and these differences could be important for drought tolerance in barley. 

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2. From the depths of the Java Trench: genomic analysis of Priestia flexa JT4 reveals bioprospecting and lycopene production potential

   https://doi.org/10.1186/s12864-024-11115-2  

   Radjasa, Ocky K; Steven, Ray; Natanael, Yosua; Nugrahapraja, Husna; Radjasa, Septhy K; Kristianti, Tati; Moeis, Maelita R; Trinugroho, Joko P; Suharya, Haekal B; Rachmatsyah, Alfito O; et al (December 2024, BMC Genomics)

   NA (Ed.)

   Cold stress in winter is one of the most severe abiotic stresses on plant growth and flourishing, and the selection of cold tolerant genotypes is an important strategy to ensure the safety of plant growth and development. Cyclocarya paliurus, a diclinous and versatile tree species originally in subtropical regions, has been introduced and cultivated in the warm temperate zone of China to meet the increasing market demand for its leaf yield. However, information regarding its cold tolerance remains limited. Based on the ploidy identification of tested materials, an imitation experiment was conducted to investigate the variation in freezing injury index and expression of the CpaWRKY family members in diploid and tetraploid C. paliurus seedlings. The results indicated a significant difference in freezing injury index between diploids and tetraploids under the imitating temperature of southern warm temperate zone, with diploids showing better cold tolerance than the tetraploids. A total of 88 CpaWRKY genes were identified from the C. paliurus genome, and RNA-Seq results showed significant differences in WRKY gene expression in C. paliurus under cold stress. Correlation analysis between differentially expressed genes and freezing injury index suggested that CpaWRKY14, CpaWRKY26 and CpaWRKY86 play essential roles in the diploids to respond to cold stress. In contrast, the major genes involved in the cold stress response in tetraploids were CpaWRKY14, CpaWRKY60, CpaWRKY63 and CpaWRKY81. Moreover, CpaWRKY14 expression was considerably higher in diploids compared to tetraploids. The results from this study not only enhance our comprehension of the role of the CpaWRKY genes in cold stress, but also provide a foundation for the genetic improvement of C. paliurus. 

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3. Molecular mapping and characterization of QTLs for grain quality traits in a RIL population of US rice under high nighttime temperature stress

   https://doi.org/10.1038/s41598-023-31399-w  

   Kumar, Anuj; Thomas, Julie; Gill, Navdeep; Dwiningsih, Yheni; Ruiz, Charles; Famoso, Adam; Pereira, Andy (December 2023, Scientific Reports)

   Abstract Elevated nighttime temperatures resulting from climate change significantly impact the rice crop worldwide. The rice ( Oryza sativa L.) plant is highly sensitive to high nighttime temperature (HNT) during grain-filling (reproductive stage). HNT stress negatively affects grain quality traits and has a major impact on the value of the harvested rice crop. In addition, along with grain dimensions determining rice grain market classes, the grain appearance and quality traits determine the rice grain market value. During the last few years, there has been a major concern for rice growers and the rice industry over the prevalence of rice grains opacity and the reduction of grain dimensions affected by HNT stress. Hence, the improvement of heat-stress tolerance to maintain grain quality of the rice crop under HNT stress will bolster future rice value in the market. In this study, 185 F 12 - recombinant inbred lines (RILs) derived from two US rice cultivars, Cypress (HNT-tolerant) and LaGrue (HNT-sensitive) were screened for the grain quality traits grain length (GL), grain width (GW), and percent chalkiness (%chalk) under control and HNT stress conditions and evaluated to identify the genomic regions associated with the grain quality traits. In total, there were 15 QTLs identified; 6 QTLs represented under control condition explaining 3.33% to 8.27% of the phenotypic variation, with additive effects ranging from − 0.99 to 0.0267 on six chromosomes and 9 QTLs represented under HNT stress elucidating 6.39 to 51.53% of the phenotypic variation, with additive effects ranging from − 8.8 to 0.028 on nine chromosomes for GL, GW, and % chalk. These 15 QTLs were further characterized and scanned for natural genetic variation in a japonica diversity panel (JDP) to identify candidate genes for GL, GW, and %chalk. We found 6160 high impact single nucleotide polymorphisms (SNPs) characterized as such depending on their type, region, functional class, position, and proximity to the gene and/or gene features, and 149 differentially expressed genes (DEGs) in the 51 Mbp genomic region comprising of the 15 QTLs. Out of which, 11 potential candidate genes showed high impact SNP associations. Therefore, the analysis of the mapped QTLs and their genetic dissection in the US grown Japonica rice genotypes at genomic and transcriptomic levels provide deep insights into genetic variation beneficial to rice breeders and geneticists for understanding the mechanisms related to grain quality under heat stress in rice. 

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4. Network‐based feature selection reveals substructures of gene modules responding to salt stress in rice

   https://doi.org/10.1002/pld3.154  

   Du, Qian; Campbell, Malachy; Yu, Huihui; Liu, Kan; Walia, Harkamal; Zhang, Qi; Zhang, Chi (August 2019, Plant Direct)

   Abstract Rice, an important food resource, is highly sensitive to salt stress, which is directly related to food security. Although many studies have identified physiological mechanisms that confer tolerance to the osmotic effects of salinity, the link between rice genotype and salt tolerance is not very clear yet. Association of gene co‐expression network and rice phenotypic data under stress has penitential to identify stress‐responsive genes, but there is no standard method to associate stress phenotype with gene co‐expression network. A novel method for integration of gene co‐expression network and stress phenotype data was developed to conduct a system analysis to link genotype to phenotype. We applied aLASSO‐based method to the gene co‐expression network of rice with salt stress to discover key genes and their interactions for salt tolerance‐related phenotypes. Submodules in gene modules identified from the co‐expression network were selected by theLASSOregression, which establishes a linear relationship between gene expression profiles and physiological responses, that is, sodium/potassium condenses under salt stress. Genes in these submodules have functions related to ion transport, osmotic adjustment, and oxidative tolerance. We argued that these genes in submodules are biologically meaningful and useful for studies on rice salt tolerance. This method can be applied to other studies to efficiently and reliably integrate co‐expression network and phenotypic data. 

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5. Time of day and genotype sensitivity adjust molecular responses to temperature stress in sorghum

   https://doi.org/10.1111/tpj.16467  

   Bonnot, Titouan; Somayanda, Impa; Jagadish, S. V. Krishna; Nagel, Dawn H. (September 2023, The Plant Journal)

   SUMMARY Sorghum is one of the four major C4 crops that are considered to be tolerant to environmental extremes. Sorghum shows distinct growth responses to temperature stress depending on the sensitivity of the genetic background. About half of the transcripts in sorghum exhibit diurnal rhythmic expressions emphasizing significant coordination with the environment. However, an understanding of how molecular dynamics contribute to genotype‐specific stress responses in the context of the time of day is not known. We examined whether temperature stress and the time of day impact the gene expression dynamics in thermo‐sensitive and thermo‐tolerant sorghum genotypes. We found that time of day is highly influencing the temperature stress responses, which can be explained by the rhythmic expression of most thermo‐responsive genes. This effect is more pronounced in thermo‐tolerant genotypes, suggesting a stronger regulation of gene expression by the time of day and/or by the circadian clock. Genotypic differences were mostly observed on average gene expression levels, which may be responsible for contrasting sensitivities to temperature stress in tolerant versus susceptible sorghum varieties. We also identified groups of genes altered by temperature stress in a time‐of‐day and genotype‐specific manner. These include transcriptional regulators and several members of the Ca²⁺‐binding EF‐hand protein family. We hypothesize that expression variation of these genes between genotypes along with time‐of‐day independent regulation may contribute to genotype‐specific fine‐tuning of thermo‐responsive pathways. These findings offer a new opportunity to selectively target specific genes in efforts to develop climate‐resilient crops based on their time‐of‐day and genotype variation responses to temperature stress. 

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