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1. Description of two cultivated and two uncultivated new Salinibacter species, one named following the rules of the bacteriological code: Salinibacter grassmerensis sp. nov.; and three named following the rules of the SeqCode: Salinibacter pepae sp. nov., Salinibacter abyssi sp. nov., and Salinibacter pampae sp. nov.

   https://doi.org/10.1016/j.syapm.2023.126416  

   Viver, Tomeu ; Conrad, Roth E. ; Lucio, Marianna ; Harir, Mourad ; Urdiain, Mercedes ; Gago, Juan F. ; Suárez-Suárez, Ana ; Bustos-Caparros, Esteban ; Sanchez-Martinez, Rodrigo ; Mayol, Eva ; et al ( May 2023 , Systematic and Applied Microbiology)
2. Phylogenomic analysis of the genus Rosenbergiella and description of Rosenbergiella gaditana sp. nov., Rosenbergiella metrosideri sp. nov., Rosenbergiella epipactidis subsp. epipactidis subsp. nov., Rosenbergiella epipactidis subsp. californiensis subsp. nov., Rosenbergiella epipactidis subsp. japonicus subsp. nov., Rosenbergiella nectarea subsp. nectarea subsp. nov. and Rosenbergiella nectarea subsp. apis subsp. nov., isolated from floral nectar and insects

   https://doi.org/10.1099/ijsem.0.005777  

   Álvarez-Pérez, Sergio ; de Vega, Clara ; Vanoirbeek, Kristof ; Tsuji, Kaoru ; Jacquemyn, Hans ; Fukami, Tadashi ; Michiels, Chris ; Lievens, Bart ( March 2023 , International Journal of Systematic and Evolutionary Microbiology)

   The genus Rosenbergiella is one of the most frequent bacterial inhabitants of flowers and a usual member of the insect microbiota worldwide. To date, there is only one publicly available Rosenbergiella genome, corresponding to the type strain of Rosenbergiella nectarea (8N4 T ), which precludes a detailed analysis of intra-genus phylogenetic relationships. In this study, we obtained draft genomes of the type strains of the other Rosenbergiella species validly published to date ( R. australiborealis , R. collisarenosi and R. epipactidis ) and 23 additional isolates of flower and insect origin. Isolate S61 T , retrieved from the nectar of an Antirrhinum sp. flower collected in southern Spain, displayed low average nucleotide identity (ANI) and in silico DNA–DNA hybridization (isDDH) values when compared with other Rosenbergiella members (≤86.5 and ≤29.8 %, respectively). Similarly, isolate JB07 T , which was obtained from the floral nectar of Metrosideros polymorpha plants in Hawaii (USA) had ≤95.7 % ANI and ≤64.1 % isDDH with other Rosenbergiella isolates. Therefore, our results support the description of two new Rosenbergiella species for which we propose the names Rosenbergiella gaditana sp. nov. (type strain: S61 T =NCCB 100789 T =DSM 111181 T ) and Rosenbergiella metrosideri sp. nov. (JB07 T =NCCB 100888 T =LMG 32616 T ). Additionally, some R. epipactidis and R. nectarea isolates showed isDDH values<79 % with other conspecific isolates, which suggests that these species include subspecies for which we propose the names Rosenbergiella epipactidis subsp. epipactidis subsp. nov. (S256 T =CECT 8502 T =LMG 27956 T ), Rosenbergiella epipactidis subsp. californiensis subsp. nov. (FR72 T =NCCB 100898 T =LMG 32786 T ), Rosenbergiella epipactidis subsp. japonicus subsp. nov. (K24 T =NCCB 100924 T =LMG 32785 T ), Rosenbergiella nectarea subsp. nectarea subsp. nov. (8N4 T = DSM 24150 T = LMG 26121 T ) and Rosenbergiella nectarea subsp. apis subsp. nov. (B1A T =NCCB 100810 T = DSM 111763 T ), respectively. Finally, we present the first phylogenomic analysis of the genus Rosenbergiella and update the formal description of the species R. australiborealis , R. collisarenosi , R. epipactidis and R. nectarea based on new genomic and phenotypic information. 

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3. Acinetobacter pollinis sp. nov., Acinetobacter baretiae sp. nov. and Acinetobacter rathckeae sp. nov., isolated from floral nectar and honey bees

   https://doi.org/10.1099/ijsem.0.004783  

   Alvarez-Perez, Sergio ; Baker, Lydia J. ; Morris, Megan M. ; Tsuji, Kaoru ; Sanchez, Vivianna A. ; Fukami, Tadashi ; Vannette, Rachel L. ; Lievens, Bart ; Hendry, Tory A. ( May 2021 , International Journal of Systematic and Evolutionary Microbiology)

   null (Ed.)

   A detailed evaluation of eight bacterial isolates from floral nectar and animal visitors to flowers shows evidence that they represent three novel species in the genus Acinetobacter . Phylogenomic analysis shows the closest relatives of these new isolates are Acinetobacter apis , Acinetobacter boissieri and Acinetobacter nectaris , previously described species associated with floral nectar and bees, but high genome-wide sequence divergence defines these isolates as novel species. Pairwise comparisons of the average nucleotide identity of the new isolates compared to known species is extremely low (<83 %), thus confirming that these samples are representative of three novel Acinetobacter species, for which the names Acinetobacter pollinis sp. nov., Acinetobacter baretiae sp. nov. and Acinetobacter rathckeae sp. nov. are proposed. The respective type strains are SCC477 T (=TSD-214 T =LMG 31655 T ), B10A T (=TSD-213 T =LMG 31702 T ) and EC24 T (=TSD-215 T =LMG 31703 T =DSM 111781 T ). 

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4. Halobacterium bonnevillei sp. nov., Halobaculum saliterrae sp. nov. and Halovenus carboxidivorans sp. nov., three novel carbon monoxide-oxidizing Halobacteria from saline crusts and soils

   https://doi.org/10.1099/ijsem.0.004282  

   Myers, Marisa R. ; King, G.M. ( July 2020 , International Journal of Systematic and Evolutionary Microbiology)

   Three novel carbon monoxide-oxidizing Halobacteria were isolated from Bonneville Salt Flats (Utah, USA) salt crusts and nearby saline soils. Phylogenetic analysis of 16S rRNA gene sequences revealed that strains PCN9 T , WSA2 T and WSH3 T belong to the genera Halobacterium , Halobaculum and Halovenus , respectively. Strains PCN9 T , WSA2 T and WSH3 T grew optimally at 40 °C (PCN9 T ) or 50 °C (WSA2 T , WSH3 T ). NaCl optima were 3 M (PCN9 T , WSA2 T ) or 4 M NaCl (WSH3 T ). Carbon monoxide was oxidized by all isolates, each of which contained a molybdenum-dependent CO dehydrogenase. G+C contents for the three respective isolates were 66.75, 67.62, and 63.97 mol% as derived from genome analyses. The closest phylogenetic relatives for PCN9 T , WSA2 T and WSH3 T were Halobacterium noricense A1 T , Halobaculum roseum D90 T and Halovenus aranensis EB27 T with 98.71, 98.19 and 95.95 % 16S rRNA gene sequence similarities, respectively. Genome comparisons of PCN9 T with Halobacterium noricense A1 T yielded an average nucleotide identity (ANI) of 82.0% and a digital DNA–DNA hybridization (dDDH) value of 25.7 %; comparisons of WSA2 T with Halobaculum roseum D90 T yielded ANI and dDDH values of 86.34 and 31.1 %, respectively. The ANI value for a comparison of WSH3 T with Halovenus aranensis EB27 T was 75.2 %. Physiological, biochemical, genetic and genomic characteristics of PCN9 T , WSA2 T and WSH3 T differentiated them from their closest phylogenetic neighbours and indicated that they represent novel species for which the names Halobaculum bonnevillei , Halobaculum saliterrae and Halovenus carboxidivorans are proposed, respectively. The type strains are PCN9 T (=JCM 32472=LMG 31022=ATCC TSD-126), WSA2 T (=JCM 32473=ATCC TSD-127) and WSH3 T (=JCM 32474=ATCC TSD-128). 

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5. Coniochaeta elegans sp. nov., Coniochaeta montana sp. nov. and Coniochaeta nivea sp. nov., three new species of endophytes with distinctive morphology and functional traits

   https://doi.org/10.1099/ijsem.0.005003  

   Arnold, A. Elizabeth ; Harrington, Alison H. ; Huang, Yu-Ling ; U'Ren, Jana M. ; Massimo, Nicholas C. ; Knight-Connoni, Victoria ; Inderbitzin, Patrik ( November 2021 , International Journal of Systematic and Evolutionary Microbiology)

   A growing interest in fungi that occur within symptom-less plants and lichens (endophytes) has uncovered previously uncharacterized species in diverse biomes worldwide. In many temperate and boreal forests, endophytic Coniochaeta (Sacc.) Cooke ( Coniochaetaceae , Coniochaetales, Sordariomycetes , Ascomycota ) are commonly isolated on standard media, but rarely are characterized. We examined 26 isolates of Coniochaeta housed at the Gilbertson Mycological Herbarium. The isolates were collected from healthy photosynthetic tissues of conifers, angiosperms, mosses and lichens in Canada, Sweden and the United States. Their barcode sequences (nuclear ribosomal internal transcribed spacer and 5.8S; ITS rDNA) were ≤97% similar to any documented species available through GenBank. Phylogenetic analyses based on two loci (ITS rDNA and translation elongation factor 1-alpha) indicated that two isolates represented Coniochaeta cymbiformispora , broadening the ecological niche and geographic range of a species known previously from burned soil in Japan. The remaining 24 endophytes represented three previously undescribed species that we characterize here: Coniochaeta elegans sp. nov., Coniochaeta montana sp. nov. and Coniochaeta nivea sp. nov. Each has a wide host range, including lichens, bryophytes and vascular plants. C. elegans sp. nov. and C. nivea sp. nov. have wide geographic ranges. C. montana sp. nov. occurs in the Madrean biome of Arizona (USA), where it is sympatric with the other species described here. All three species display protease, chitinase and cellulase activity in vitro . Overall, this study provides insight into the ecological and evolutionary diversity of Coniochaeta and suggests that these strains may be amenable for studies of traits relevant to a horizontally transmitted, symbiotic lifestyle. 

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