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Title: Biomanufacturing of organ-specific tissues with high cellular density and embedded vascular channels
Engineering organ-specific tissues for therapeutic applications is a grand challenge, requiring the fabrication and maintenance of densely cellular constructs composed of ~10 8 cells/ml. Organ building blocks (OBBs) composed of patient-specific–induced pluripotent stem cell–derived organoids offer a pathway to achieving tissues with the requisite cellular density, microarchitecture, and function. However, to date, scant attention has been devoted to their assembly into 3D tissue constructs. Here, we report a biomanufacturing method for assembling hundreds of thousands of these OBBs into living matrices with high cellular density into which perfusable vascular channels are introduced via embedded three-dimensional bioprinting. The OBB matrices exhibit the desired self-healing, viscoplastic behavior required for sacrificial writing into functional tissue (SWIFT). As an exemplar, we created a perfusable cardiac tissue that fuses and beats synchronously over a 7-day period. Our SWIFT biomanufacturing method enables the rapid assembly of perfusable patient- and organ-specific tissues at therapeutic scales.  more » « less
Award ID(s):
1647837
PAR ID:
10134061
Author(s) / Creator(s):
; ; ; ; ; ;
Date Published:
Journal Name:
Science Advances
Volume:
5
Issue:
9
ISSN:
2375-2548
Page Range / eLocation ID:
eaaw2459
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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