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Title: Analysis of Single-Cell Gene Pair Coexpression Landscapes by Stochastic Kinetic Modeling Reveals Gene-Pair Interactions in Development
Award ID(s):
1763272
NSF-PAR ID:
10146850
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Frontiers in Genetics
Volume:
10
ISSN:
1664-8021
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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  1. Abstract

    Hybridization, introgression, and reciprocal gene flow during speciation, specifically the generation of mitonuclear discordance, are increasingly observed as parts of the speciation process. Genomic approaches provide insight into where, when, and how adaptation operates during and after speciation and can measure historical and modern introgression. Whether adaptive or neutral in origin, hybridization can cause mitonuclear discordance by placing the mitochondrial genome of one species (or population) in the nuclear background of another species. The latter, introgressed species may eventually have its own mtDNA replaced or “captured” by other species across its entire geographical range. Intermediate stages in the capture process should be observable. Two nonsister species of Australasian monarch‐flycatchers, Spectacled Monarch (Symposiachrus trivirgatus) mostly of Australia and Indonesia and Spot‐winged Monarch (S. guttula) of New Guinea, present an opportunity to observe this process. We analysed thousands of single nucleotide polymorphisms (SNPs) derived from ultraconserved elements of all subspecies of both species. Mitochondrial DNA sequences of Australian populations ofS. trivirgatusform two paraphyletic clades, one being sister to and presumably introgressed byS. guttuladespite little nuclear signal of introgression. Population genetic analyses (e.g., tests for modern and historical gene flow and selection) support at least one historical gene flow event betweenS. guttulaand AustralianS. trivirgatus. We also uncovered introgression from the Maluku Islands subspecies ofS. trivirgatusinto an island population ofS. guttula, resulting in apparent nuclear paraphyly. We find that neutral demographic processes, not adaptive introgression, are the most likely cause of these complex population histories. We suggest that a Pleistocene extinction ofS. guttulafrom mainland Australia resulted from range expansion byS. trivirgatus.

     
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  2. A bstract A search for new heavy resonances decaying to a pair of Higgs bosons (HH) in proton-proton collisions at a center-of-mass energy of 13 TeV is presented. Data were collected with the CMS detector at the LHC in 2016–2018, corresponding to an integrated luminosity of 138 fb − 1 . Resonances with a mass between 0.8 and 4.5 TeV are considered using events in which one Higgs boson decays into a bottom quark pair and the other into final states with either one or two charged leptons. Specifically, the single-lepton decay channel $$ \mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}{\mathrm{WW}}^{\ast}\to \mathrm{b}\overline{\mathrm{b}}\ell v\mathrm{q}{\overline{\mathrm{q}}}^{\prime } $$ HH → b b ¯ WW ∗ → b b ¯ ℓ v q q ¯ ′ and the dilepton decay channels $$ \mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}{\mathrm{WW}}^{\ast}\to \mathrm{b}\overline{\mathrm{b}}\ell v\ell v $$ HH → b b ¯ WW ∗ → b b ¯ ℓ v ℓ v and $$ \mathrm{HH}\to \mathrm{b}\overline{\mathrm{b}}\uptau \uptau \to \mathrm{b}\overline{\mathrm{b}}\ell vv\ell vv $$ HH → b b ¯ ττ → b b ¯ ℓ vv ℓ vv are examined, where ℓ in the final state corresponds to an electron or muon. The signal is extracted using a two-dimensional maximum likelihood fit of the $$ \mathrm{H}\to \mathrm{b}\overline{\mathrm{b}} $$ H → b b ¯ jet mass and HH invariant mass distributions. No significant excess above the standard model expectation is observed in data. Model-independent exclusion limits are placed on the product of the cross section and branching fraction for narrow spin-0 and spin-2 massive bosons decaying to HH. The results are also interpreted in the context of radion and bulk graviton production in models with a warped extra spatial dimension. The results provide the most stringent limits to date for X → HH signatures with final-state leptons and at some masses provide the most sensitive limits of all X → HH searches. 
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