Understanding the Coacervate-to-Vesicle Transition of Globular Fusion Proteins to Engineer Protein Vesicle Size and Membrane Heterogeneity
- Award ID(s):
- 1709428
- NSF-PAR ID:
- 10184608
- Date Published:
- Journal Name:
- Biomacromolecules
- Volume:
- 20
- Issue:
- 9
- ISSN:
- 1525-7797
- Page Range / eLocation ID:
- 3494 to 3503
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
More Like this
-
Neuroblastoma cells are often used as a cell model to study Parkinson's disease, which causes reduced dopamine release in substantia nigra, the midbrain that controls movements. In this paper, we developed a 1024-ch monolithic CMOS sensor array that has the spatiotemporal resolution as well as low-noise performance to monitor single vesicle release of dopamine from neuroblastoma cells. The CMOS device integrates 1024 on-chip electrodes with an individual size of 15 μm × 15 μm and 1024 transimpedance amplifiers for each electrode, which are each capable of measuring sub-pA current. Thus, this device can be used to study the detailed molecular dynamics of dopamine secretion at single vesicle resolution.more » « less