Most undergraduates studying biochemistry and molecular biology get their broadest exposure to wet-lab techniques in protein and nucleic acid chemistry (and, increasingly, computer/visualization) in their upper-level laboratory courses. These tend to be juniors and seniors with well-defined career goals. Some of these students will have already have a research background in a traditional one-to-one (or one-to-few) research mentoring setting, for example a summer research program. This approach has proved effective at increasing student learning and persistence in the sciences. At the same time, extended full-time PI-directed research is limited in the number of students served, and can even present a barrier. To broaden the impact of teaching through research, many practitioners have adopted a CURE, or Course-based Undergraduate Research Experience, approach.This presentation reports on “BASIL” (Biochemical Authentic Scientific Inquiry Laboratory), a team of faculty who have worked to bring computational and wet-lab protein science to the biochemistry teaching lab. Together, we have developed a protein biochemistry CURE to determine enzymatic function of proteins of unknown activity. This work leverages the results of the Protein Structure Initiative, a fifteen-year NIH-funded effort which concluded in 2015 with the publication and distribution of more than 5000 previously uncharacterized proteins. The great majority of these are “orphans,” with high quality structures and pre-cloned expression plasmids available, but no research on their enzymatic function or role in native organisms. The BASIL consortium of undergraduate biochemistry faculty and students seeks to identify functional properties of a subset of these uncharacterized proteins, seeking to unify structure and function relationships. Currently, implementable modules are available for faculty who wish to adopt them, and expected student results will be presented.Support or Funding InformationSupported by NSF IUSE 1709278This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal.
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Responses to the COVID-19 Pandemic by the Biochemistry Authentic Scientific Inquiry Lab (BASIL) CURE Consortium: Reflections and a Case Study on the Switch to Remote Learning
Campus shutdowns during the SARS-CoV2 pandemic posed unique challenges to faculty and students engaged in laboratory courses. Formerly hands-on experiments had to be quickly pivoted to emergency remote learning. While some resources existed prior to this period, many currently available online modules and/or simulations focus on a single technique. The Biochemistry Authentic Scientific Inquiry Lab (BASIL) curriculum has, for several years, provided a robust, linked, holistic inquiry experience that allows students to make connections between multiple techniques, both computational in nature as well as wet-lab based. As a Course-based Undergraduate Research Experience (CURE), this flexible, module-based curriculum allows students to generate original hypotheses based on analysis of proteins of unknown function. We have taught this curriculum as the upper-level laboratory course on our campuses and were obliged to transition to remote instruction at various points in the course sequence. We report on the experiences of faculty and students over the transition period in this course. Additionally, we report as a case study results of one of our campus’ ongoing discipline-based education research (DBER) on the BASIL curriculum prior to and during remote delivery.
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- NSF-PAR ID:
- 10184725
- Date Published:
- Journal Name:
- Journal of Chemical Education
- ISSN:
- 0021-9584
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Abstract Course‐based Undergraduate Research Experiences (CUREs) can be a very effective means to introduce a large number of students to research. CUREs are often an extension of the instructor's research, which may make them difficult to replicate in other settings because of differences in expertise or facilities. The BASIL (Biochemistry Authentic Scientific Inquiry Lab) CURE has evolved over the past 4 years as faculty members with different backgrounds, facilities, and campus cultures have all contributed to a robust curriculum focusing on enzyme function prediction that is suitable for implementation in a wide variety of academic settings. © 2019 International Union of Biochemistry and Molecular Biology, 47(5):498–505, 2019.
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Undergraduate instructional biology laboratories are typically taught within two paradigms. Some labs focus on protocols and techniques delivered in “cookbook” format with defined experimental outcomes. There is increasing momentum to alternatively employ student-driven, open-ended, and discovery-based strategies, often
via course-based undergraduate research experiences (CUREs) using crowd-sourcing initiatives. A fraction of students also participate in funded research in faculty research labs, where they have opportunities to work on projects designed to expand the frontiers of human knowledge. These experiences are widely recognized as valuable but are not scalable, as most institutions have many more undergraduates than research lab positions. We sought to address this gap through our department’s curriculum by creating an opportunity for students to participate in the real-world research process within a laboratory course. We conceived, developed, and delivered an authentic, guided research experience to students in an upper-level molecular biology laboratory course. We refer to this model as a “research program-linked CURE.” The research questions come directly from a faculty member’s research lab and evolve along with that research program. Students study post-transcriptional regulation in mycobacteria. We use current molecular biology methodologies to test hypotheses like “UTRs affect RNA and protein expression levels,” “there is functional redundancy among RNA helicases,” and “carbon starvation alters mRNA 5′ end chemistries.” We conducted standard assessments and developed a customized “Skills and Concepts Inventory” survey to gauge how well the course met our student learning outcomes. We report the results of our assessments and describe challenges addressed during development and execution of the course, including organizing activities to fit within an instructional lab, balancing breadth with depth, and maintaining authenticity while giving students the experience of obtaining interpretable and novel results. Our data suggest student learning was enhanced through this truly authentic research approach. Further, students were able to perceive they were participants and contributors within an active research paradigm. Students reported increases in their self-identification as scientists, and a positive impact on their career trajectories. An additional benefit was reciprocation back to the funded research laboratory, by funneling course alumni, results, materials, and protocols. -
Course-based Undergraduate Research Experiences (CUREs) are an increasingly utilized model for exposing students to research. The lack of robust assessments is a major hurdle to wider adoption of CUREs. The Coronavirus Infectious Disease 2019 (COVID-19) pandemic necessitated a drastic shift of in-person courses to the online format. Using the Participant Perception Indicator (PPI) survey, we measured students’ self-reported changes in learning from such a biochemistry course at a large university in south Florida based on the Biochemistry Authentic Scientific Inquiry Lab (BASIL) model. By doing this, we were able to better understand the student-benefits of CUREs and how these benefits are affected by changes in learning modalities between two relevant semesters, i.e., winter and summer of 2020. Anticipated learning outcomes (ALOs) help partially fill the gap left by the loss of physical interaction in experimental procedures. Our analysis indicated that students learned more through bioinformatic experiments compared to their wet-lab counterparts. Using pre- and post- surveys, students reported that their experience and confidence gains lagged behind their knowledge gain of technique-based skills. Students are not as confident in their understanding of techniques when unable to perform those in the physical laboratory. Thus, despite extensive pursuit of the purpose and protocols of the experiments and techniques, neither their experience nor their confidence was on par with their knowledge. This study is one of the first examples demonstrating a quantitative student-learning assessment of a CURE in the science, technology, engineering, and mathematics (STEM) disciplines. The novel assessment strategies targeted to identify gaps in learning mastery could facilitate the adoption of CUREs, fostering opportunities for all undergraduate students to vital laboratory research experiences in STEM.more » « less
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Evidence has shown that facilitating student-centered learning (SCL) in STEM classrooms enhances student learning and satisfaction [1]–[3]. However, despite increased support from educational and government bodies to incorporate SCL practices [1], minimal changes have been made in undergraduate STEM curriculum [4]. Faculty often teach as they were taught, relying heavily on traditional lecture-based teaching to disseminate knowledge [4]. Though some faculty express the desire to improve their teaching strategies, they feel limited by a lack of time, training, and incentives [4], [5]. To maximize student learning while minimizing instructor effort to change content, courses can be designed to incorporate simpler, less time-consuming SCL strategies that still have a positive impact on student experience. In this paper, we present one example of utilizing a variety of simple SCL strategies throughout the design and implementation of a 4-week long module. This module focused on introductory tissue engineering concepts and was designed to help students learn foundational knowledge within the field as well as develop critical technical skills. Further, the module sought to develop important professional skills such as problem-solving, teamwork, and communication. During module design and implementation, evidence-based SCL teaching strategies were applied to ensure students developed important knowledge and skills within the short timeframe. Lectures featured discussion-based active learning exercises to encourage student engagement and peer collaboration [6]–[8]. The module was designed using a situated perspective, acknowledging that knowing is inseparable from doing [9], and therefore each week, the material taught in the two lecture sessions was directly applied to that week’s lab to reinforce students’ conceptual knowledge through hands-on activities and experimental outcomes. Additionally, the majority of assignments served as formative assessments to motivate student performance while providing instructors with feedback to identify misconceptions and make real-time module improvements [10]–[12]. Students anonymously responded to pre- and post-module surveys, which focused on topics such as student motivation for enrolling in the module, module expectations, and prior experience. Students were also surveyed for student satisfaction, learning gains, and graduate student teaching team (GSTT) performance. Data suggests a high level of student satisfaction, as most students’ expectations were met, and often exceeded. Students reported developing a deeper understanding of the field of tissue engineering and learning many of the targeted basic lab skills. In addition to hands-on skills, students gained confidence to participate in research and an appreciation for interacting with and learning from peers. Finally, responses with respect to GSTT performance indicated a perceived emphasis on a learner-centered and knowledge/community-centered approaches over assessment-centeredness [13]. Overall, student feedback indicated that SCL teaching strategies can enhance student learning outcomes and experience, even over the short timeframe of this module. Student recommendations for module improvement focused primarily on modifying the lecture content and laboratory component of the module, and not on changing the teaching strategies employed. The success of this module exemplifies how instructors can implement similar strategies to increase student engagement and encourage in-depth discussions without drastically increasing instructor effort to re-format course content. Introduction.more » « less
- Free Publicly Accessible Full Text
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Accepted Manuscript1.0
- Journal Article:
- https://doi.org/10.1021/acs.jchemed.0c00729
