Cleavage under targets and release using nuclease (CUT&RUN) is a recently developed chromatin profiling technique that uses a targeted micrococcal nuclease cleavage strategy to obtain high‐resolution binding profiles of protein factors or to map histones with specific post‐translational modifications. Due to its high sensitivity, CUT&RUN allows quality binding profiles to be obtained with only a fraction of the starting material and sequencing depth typically required for other chromatin profiling techniques such as chromatin immunoprecipitation. Although CUT&RUN has been widely adopted in multiple model systems, it has rarely been utilized in
- Award ID(s):
- 1703394
- NSF-PAR ID:
- 10190897
- Date Published:
- Journal Name:
- Micromachines
- Volume:
- 11
- Issue:
- 1
- ISSN:
- 2072-666X
- Page Range / eLocation ID:
- 99
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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