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1. Sporogenesis in Physcomitrium patens: Intergenerational collaboration and the development of the spore wall and aperture

   https://doi.org/10.3389/fcell.2023.1165293  

   Renzaglia, Karen S. ; Ashton, Neil W. ; Suh, Dae-Yeon ( April 2023 , Frontiers in Cell and Developmental Biology)

   Although the evolution of spores was critical to the diversification of plants on land, sporogenesis is incompletely characterized for model plants such as Physcomitrium patens . In this study, the complete process of P. patens sporogenesis is detailed from capsule expansion to mature spore formation, with emphasis on the construction of the complex spore wall and proximal aperture. Both diploid (sporophytic) and haploid (spores) cells contribute to the development and maturation of spores. During capsule expansion, the diploid cells of the capsule, including spore mother cells (SMCs), inner capsule wall layer (spore sac), and columella, contribute a locular fibrillar matrix that contains the machinery and nutrients for spore ontogeny. Nascent spores are enclosed in a second matrix that is surrounded by a thin SMC wall and suspended in the locular material. As they expand and separate, a band of exine is produced external to a thin foundation layer of tripartite lamellae. Dense globules assemble evenly throughout the locule, and these are incorporated progressively onto the spore surface to form the perine external to the exine. On the distal spore surface, the intine forms internally, while the spiny perine ornamentation is assembled. The exine is at least partially extrasporal in origin, while the perine is derived exclusively from outside the spore. Across the proximal surface of the polar spores, an aperture begins formation at the onset of spore development and consists of an expanded intine, an annulus, and a central pad with radiating fibers. This complex aperture is elastic and enables the proximal spore surface to cycle between being compressed (concave) and expanded (rounded). In addition to providing a site for water intake and germination, the elastic aperture is likely involved in desiccation tolerance. Based on the current phylogenies, the ancestral plant spore contained an aperture, exine, intine, and perine. The reductive evolution of liverwort and hornwort spores entailed the loss of perine in both groups and the aperture in liverworts. This research serves as the foundation for comparisons with other plant groups and for future studies of the developmental genetics and evolution of spores across plants. 

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2. Infectivity of the parasite Metschnikowia bicuspidata is decreased by time spent as a transmission spore, but exposure to phycotoxins in the water column has no effect

   https://doi.org/10.1111/fwb.14082  

   Sánchez, Kristel F. ; Zhong, Baili ; Agudelo, Jorge A. ; Duffy, Meghan A. ( April 2023 , Freshwater Biology)

   Transmission from one host to another is a crucial component of parasite fitness. For some aquatic parasites, transmission occurs via a free‐living stage that spends time in the water, awaiting an encounter with a new host. These parasite transmission stages can be impacted by biotic and abiotic factors that influence the parasite's ability to successfully infect or grow in a new host.

   Here we tested whether time spent in the water column and/or exposure to common cyanobacterial toxins impacted parasite transmission stages. More specifically, we tested whether the infectivity, within host growth, and virulence of the fungal parasiteMetschnikowia bicuspidatachanged as a result of time spent in the water or from exposure to cyanotoxins in the water column. We exposed parasite transmission spores to different levels of one of two ecologically important cyanotoxins, microcystin‐LR and anatoxin‐a, and factorially manipulated the amount of time spores were incubated in water. We removed the toxins and used those same spores to infect one genotype of the common lake zooplanktonDaphnia dentifera.

   We found that cyanotoxins did not impact parasite fitness (infection prevalence and spore yield per infected host) or virulence (host lifetime reproduction and survivorship) at the tested concentrations (10 and 30 μg/L). However, we found that spending longer as a transmission spore decreased a spore's chances for successful infection: spores that were only incubated for 24 hr infected approximately 75% of exposed hosts, whereas spores incubated for 10 days infected less than 50% of exposed hosts.

   We also found a negative relationship between the final spore yield from infected hosts and the proportion of hosts that became infected. In treatments where spores spent longer in the water column prior to encountering a host, infection prevalence was lower (indicating lower per spore infectivity), but each infected host yielded more spores at the end of infection. We hypothesise that this pattern may result from intraspecific parasite competition within the host.

   Overall, these results suggest that transmission spores of this parasite are not strongly influenced by cyanotoxins in the water column, but that other aspects of spending time in the water strongly influence parasite fitness.

    

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3. Radar imaging of fractures and voids behind the walls of an underground mine

   https://doi.org/10.1190/geo2020-0763.1  

   Abbasi Baghbadorani, Amin ; Hole, John A. ; Baggett, Jonathan ; Ripepi, Nino ( July 2021 , GEOPHYSICS)

   Two- and three-dimensional rock-penetrating-radar data were acquired on the wall of a pillar in an underground limestone mine. The objective was to test the ability of radar to image fractures and karst voids and to characterize their geometry, aperture, and fluid content, with the goal of mitigating mining hazards. Strong radar reflections in the field data correlate with fractures and a cave exposed on the pillar walls. Large pillar wall topography was included in the steep-dip Kirchhoff migration algorithm because standard elevation corrections are inaccurate. The depth-migrated 250 MHz radar images illuminate fractures, karst voids, and the far wall of the pillar up to approximately 25 m depth into the rock, with a spatial resolution of <0.5 m. Higher frequency radar improved the image resolution and aided in the interpretation, but at the cost of shallower depth of penetration and extra acquisition effort. Due to the strong contrast in physical properties between the rock and the fracture fluid, fractures with apertures as thin as a 50th of a radar wavelength were imaged. Water-filled fractures with mm-scale aperture and air-filled fractures with cm-scale apertures produce strong reflections at 250 MHz. A strong variation in the reflection amplitude along each fracture is interpreted to represent the variable fracture aperture and the nonplanar fracture structure. Fracture apertures were quantitatively measured, but distinguishing water from air-filled fractures was not possible due to the complex radar wavelet and fracture geometry. Two conjugate fracture sets were imaged. One of these fracture sets dominates the rock mass stability and water inrush challenges throughout the mine. All of the detected voids and a large cave are at the intersection of two fractures, indicating preferential water flow and dissolution along conjugate fracture intersections. Detecting, locating, and characterizing fractures and voids prior to excavation can enable miners to mitigate potential collapse and flood hazards before they occur. 

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4. Dynamic aqueous transformations of lithium cobalt oxide nanoparticle induce distinct oxidative stress responses of B. subtilis

   https://doi.org/10.1039/D0EN01151G  

   Gari, Metti K. ; Lemke, Paul ; Lu, Kelly H. ; Laudadio, Elizabeth D. ; Henke, Austin H. ; Green, Curtis M. ; Pho, Thomas ; Hoang, Khoi Nguyen ; Murphy, Catherine J. ; Hamers, Robert J. ; et al ( January 2021 , Environmental Science: Nano)

   null (Ed.)

   Lithium cobalt oxide (LiCoO 2 ), an example of nanoscale transition metal oxide and a widely commercialized cathode material in lithium ion batteries, has been shown to induce oxidative stress and generate intracellular reactive oxygen species (ROS) in model organisms. In this study, we aimed to understand the time-dependent roles of abiotic ROS generation and Co ions released in aqueous medium by LiCoO 2 NPs, and examined the induced biological responses in model bacterium, B. subtilis upon exposure. We found that the redox-active LiCoO 2 NPs produced abiotic ROS primarily through H 2 O 2 generation when freshly suspended. Subsequently, the freshly-suspended LiCoO 2 NPs induced additional DNA breakage, and changes in expression of oxidative stress genes in B. subtilis that could not be accounted for by the released Co ions alone. Notably, in 48 hour old LiCoO 2 suspensions, H 2 O 2 generation subsided while higher concentrations of Co ions were released. The biological responses in DNA damage and gene expression to the aged LiCoO 2 NPs recapitulated those induced by the released Co ions. Our results demonstrated oxidative stress mechanisms for bacteria exposed to LiCoO 2 NPs were mediated by the generation of distinct biotic and abiotic ROS species, which depended on the aqueous transformation state of the NPs. This study revealed the interdependent and dynamic nature of NP transformation and their biological consequences where the state of NPs resulted in distinct NP-specific mechanisms of oxidative injury. Our work highlights the need to capture the dynamic transformation of NPs that may activate the multiple routes of oxidative stress responses in cells. 

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5. Saccharomyces spores are born prepolarized to outgrow away from spore–spore connections and penetrate the ascus wall

   https://doi.org/10.1002/yea.3540  

   Heasley, Lydia R. ; Singer, Emily ; Cooperman, Benjamin J. ; McMurray, Michael A. ( December 2020 , Yeast)

   How nonspore haploidSaccharomycescells choose sites of budding and polarize towards pheromone signals in order to mate has been a subject of intense study. Unlike nonspore haploids, sibling spores produced via meiosis and sporulation by a diploid cell are physically interconnected and encased in a sac derived from the old cell wall of the diploid, called the ascus. Nonspore haploids bud adjacent to previous sites of budding, relying on stable cortical landmarks laid down during prior divisions, but because spore membranes are made de novo, it was assumed that, as is known for fission yeast,Saccharomycesspores break symmetry and polarize at random locations. Here, we show that this assumption is incorrect:Saccharomyces cerevisiaespores are born prepolarized to outgrow, prior to budding or mating, away from interspore bridges. Consequently, when spores bud within an intact ascus, their buds locally penetrate the ascus wall, and when they mate, the resulting zygotes adopt a unique morphology reflective of repolarization towards pheromone. Long‐lived cortical foci containing the septin Cdc10 mark polarity sites, but the canonical bud site selection programme is dispensable for spore polarity, thus the origin and molecular composition of these landmarks remain unknown. These findings demand further investigation of previously overlooked mechanisms of polarity establishment and local cell wall digestion and highlight how a key step in theSaccharomyceslife cycle has been historically neglected.

    

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