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Title: Characterization of an angular domain fluorescence optical projection tomography system for mesoscopic lymph node imaging
Transmittance and fluorescence optical projection tomography can offer high-resolution and high-contrast visualization of whole biological specimens; however, applications are limited to samples exhibiting minimal light scattering. Our previous work demonstrated that angular-domain techniques permitted imaging of ∼<#comment/> 1 c m diameter noncleared lymph nodes because of their low scattering nature. Here, an angle-restricted transmittance/fluorescence system is presented and characterized in terms of geometric and fluorescence concentration reconstruction accuracy as well as spatial resolution, depth of focus, and fluorescence limits of detection. Using lymph node mimicking phantoms, results demonstrated promising detection and localization capabilities relevant for clinical lymph node applications.  more » « less
Award ID(s):
1653627
PAR ID:
10206757
Author(s) / Creator(s):
; ; ; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Applied Optics
Volume:
60
Issue:
1
ISSN:
1559-128X; APOPAI
Format(s):
Medium: X Size: Article No. 135
Size(s):
Article No. 135
Sponsoring Org:
National Science Foundation
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