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Title: One-pot synthesis of heterodimeric agonists that activate the canonical Wnt signaling pathway
Fragment antigen-binding domains (Fabs) from anti-Frizzled and anti-LRP6 monoclonal antibodies were conjugated using SpyTag–SpyCatcher chemistry via a one-pot reaction. The resulting synthetic heterodimeric agonist outperformed the natural ligand, Wnt-3a, in activating canonical Wnt signaling in mammalian cells. This approach should be broadly applicable to activate receptor-mediated cellular signaling.  more » « less
Award ID(s):
1648035
NSF-PAR ID:
10209654
Author(s) / Creator(s):
; ; ; ; ; ; ; ;
Date Published:
Journal Name:
Chemical Communications
Volume:
56
Issue:
25
ISSN:
1359-7345
Page Range / eLocation ID:
3685 to 3688
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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