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1. Cellular metabolism and IL-6 concentrations during stimulated inflammation in small and large dog breeds’ primary fibroblasts cells, as they age

   https://doi.org/10.1242/jeb.233734  

   Jimenez, Ana Gabriela ; Downs, Cynthia J. ; Lalwani, Sahil ; Cipolli, William ( January 2021 , Journal of Experimental Biology)

   The immune system undergoes marked changes during aging characterized by a state of chronic, low-grade inflammation, so called inflammaging. Domestic dogs are the most morphological and physiological diverse group of mammals, with the widest range in body masses for a single species. Additionally, smaller dogs tend to live significantly longer than larger dogs across all breeds. Body mass is intricately linked to mass-specific metabolism and aging rates, thus, dogs are exemplary for studies in inflammaging. Dermal fibroblasts cells play an important role in skin inflammation, and as such, are a good cell type to determine inflammatory patterns in dogs. Here, we examine aerobic and glycolytic cellular metabolism, and IL-6 concentrations in primary fibroblast cells isolated from small and large, young and old dogs when treated with lipopolysaccharide (LPS) from Escherichia coli to stimulate an inflammatory phenotype. We found no differences in cellular metabolism of any group when treated with LPS. Unlike mice and humans, there was a less drastic amplification of IL-6 concentration after LPS treatment in the geriatric population of dogs compared with puppies. We also found evidence that large breed puppies have significantly less background or control IL-6 concentrations compared with small breed puppies. This implies that themore »patterns of inflammaging in dogs may be distinct and different from other mammals commonly studied.« less
2. Stiffness-matched extracellular matrices comprising collagen or fibrin differentially affect cell-mediated remodeling and peri-cellular stiffness

   https://doi.org/10.21203/rs.3.rs-1348112/v1  

   Jagiełło, A. ; Castillo, U. ; Botvinick, E. ( February 2022 , Research square)

   Cells are known to continuously remodel their local extracellular matrix (ECM) and in a reciprocal way, they can also respond to mechanical and biochemical properties of their fibrous environment. In this study, we measured how stiffness around dermal fibroblasts (DFs) and human fibrosarcoma HT1080 cells differs with concentration of rat tail type 1 collagen (T1C) and type of ECM. Peri-cellular stiffness was probed in four directions using multi-axes optical tweezers active microrheology (AMR). First, we found that neither cell type significantly altered local stiffness landscape at different concentrations of T1C. Next, rat tail T1C, bovine skin T1C and fibrin cell-free hydrogels were polymerized at concentrations formulated to match median stiffness value. Each of these hydrogels exhibited distinct fiber architecture. Stiffness landscape and fibronectin secretion, but not nuclear/cytoplasmic YAP ratio differed with ECM type. Further, cell response to Y27632 or BB94 treatments, inhibiting cell contractility and activity of matrix metalloproteinases, respectively, was also dependent on ECM type. Given differential effect of tested ECMs on peri-cellular stiffness landscape, treatment effect and cell properties, this study underscores the need for peri-cellular and not bulk stiffness measurements in studies on cellular mechanotransduction.
3. Dermal fibroblasts and human breast cancer cells differentially stiffen their local matrix

   Jagiello, A ; Lim, M. ; Botvinick, E ( July 2021 , EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS)

   Bulk ECM stiffness measurements are often used in research on cell mechanobiology. However, past studies by our group have shown that peri-cellular stiffness can span few orders of magnitude and diverges from the bulk properties. Us- ing optical tweezers active microrheology (AMR) we can de- scribe stiffness landscape around individual cells. In this study, we show how different cell lines cultured in 1.0 and 1.5 mg/ml type 1 collagen (T1C) create disparate patterns of peri-cellular stiffness. Dermal fibroblasts (DFs) increase peri-cellular stiffness, when embedded in 1.0 mg/ml T1C hy- drogels, but do not alter stiffness in 1.5 mg/ml T1C hydro- gels. In contrast, invasive human breast cancer MDA-MB- 231 cells (MDAs) do not significantly change the stiffness of T1C hydrogels, as compared to cell-free controls. Results indicate that while MDAs adapt to the bulk ECM stiffness, DFs regulate local stiffness to levels they intrinsically “fa- vor”. Further, cells were also subjected to treatments that were previously shown to regulate their migration, prolifera- tion and contractility. Following each treatment, cells estab- lished dissimilar stiffness patterns. Stiffness magnitude and extent of anisotropy varied with the cell line, T1C concen- tration and treatment. In summary, we demonstrate that AMR can reveal otherwise maskedmore »mechanical properties of the local ECM, which are known to affect cell behavior.« less
4. Cells utilize strain hardening and crosslinking to establish their extracellular niche in fibrous tissue

   Jagiello, A. ; Lim, M. ; Botvinick, E. ( March 2021 , APS March Meeting 2021)

   Bulk measurements of ECM stiffness are commonly used in mechanobiology. However, peri-cellular stiffness can be quite heterogenous and divergent from the bulk properties. Here, we use optical tweezers active microrheology (AMR) to quantify how two different cell lines embedded in 1.0 and 1.5 mg/ml type 1 collagen (T1C) establish dissimilar patterns of peri-cellular stiffness. We found that dermal fibroblasts (DFs) increase local stiffness of 1.0 mg/ml T1C hydrogels, but surprisingly do not alter stiffness of 1.5 mg/ml T1C hydrogels. In contrast, MDA-MB-231 cells (MDAs) predominantly do not stiffen T1C hydrogels, as compared to cell-free controls. Results suggest that MDAs adapt to the bulk ECM stiffness, while DFs regulate local stiffness to levels they intrinsically “prefer”. Further, cells were subjected to treatments, that were previously shown to alter migration, proliferation and contractility of DFs and MDAs. Following treatment, both cell lines established different levels of stiffness magnitude and anisotropy, which were dependent on the cell line, T1C concentration and treatment. In summary, our findings demonstrate that AMR reveals otherwise masked mechanical properties such as spatial gradients and anisotropy, which are known to affect cell behavior at the macro-scale.
5. Radical chemistry in oxidation flow reactors for atmospheric chemistry research

   https://doi.org/10.1039/C9CS00766K  

   Peng, Zhe ; Jimenez, Jose L. ( May 2020 , Chemical Society Reviews)

   Environmental chambers have been playing a vital role in atmospheric chemistry research for seven decades. In last decade, oxidation flow reactors (OFR) have emerged as a promising alternative to chambers to study complex multigenerational chemistry. OFR can generate higher-than-ambient concentrations of oxidants via H 2 O, O 2 and O 3 photolysis by low-pressure-Hg-lamp emissions and reach hours to days of equivalent photochemical aging in just minutes of real time. The use of OFR for volatile-organic-compound (VOC) oxidation and secondary-organic-aerosol formation has grown very rapidly recently. However, the lack of detailed understanding of OFR photochemistry left room for speculation that OFR chemistry may be generally irrelevant to the troposphere, since its initial oxidant generation is similar to stratosphere. Recently, a series of studies have been conducted to address important open questions on OFR chemistry and to guide experimental design and interpretation. In this Review, we present a comprehensive picture connecting the chemistries of hydroxyl (OH) and hydroperoxy radicals, oxidized nitrogen species and organic peroxy radicals (RO 2 ) in OFR. Potential lack of tropospheric relevance associated with these chemistries, as well as the physical conditions resulting in it will also be reviewed. When atmospheric oxidation is dominated by OH, OFRmore »conditions can often be similar to ambient conditions, as OH dominates against undesired non-OH effects. One key reason for tropospherically-irrelevant/undesired VOC fate is that under some conditions, OH is drastically reduced while non-tropospheric/undesired VOC reactants are not. The most frequent problems are running experiments with too high precursor concentrations, too high UV and/or too low humidity. On other hand, another cause of deviation from ambient chemistry in OFR is that some tropospherically-relevant non-OH chemistry ( e.g. VOC photolysis in UVA and UVB) is not sufficiently represented under some conditions. In addition, the fate of RO 2 produced from VOC oxidation can be kept relevant to the troposphere. However, in some cases RO 2 lifetime can be too short for atmospherically-relevant RO 2 chemistry, including its isomerization. OFR applications using only photolysis of injected O 3 to generate OH are less preferable than those using both 185 and 254 nm photons (without O 3 injection) for several reasons. When a relatively low equivalent photochemical age (<∼1 d) and high NO are needed, OH and NO generation by organic-nitrite photolysis in the UVA range is preferable. We also discuss how to achieve the atmospheric relevance for different purposes in OFR experimental planning.« less