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Title: Fast, multiplane line-scan confocal microscopy using axially distributed slits
The inherent constraints on resolution, speed and field of view have hindered the development of high-speed, three-dimensional microscopy techniques over large scales. Here, we present a multiplane line-scan imaging strategy, which uses a series of axially distributed reflecting slits to probe different depths within a sample volume. Our technique enables the simultaneous imaging of an optically sectioned image stack with a single camera at frame rates of hundreds of hertz, without the need for axial scanning. We demonstrate the applicability of our system to monitor fast dynamics in biological samples by performing calcium imaging of neuronal activity in mouse brains and voltage imaging of cardiomyocytes in cardiac samples.  more » « less
Award ID(s):
1647837
PAR ID:
10213347
Author(s) / Creator(s):
; ; ; ; ; ;
Publisher / Repository:
Optical Society of America
Date Published:
Journal Name:
Biomedical Optics Express
Volume:
12
Issue:
3
ISSN:
2156-7085
Format(s):
Medium: X Size: Article No. 1339
Size(s):
Article No. 1339
Sponsoring Org:
National Science Foundation
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