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1. Thermal considerations for microswimmer trap-and-release using standing surface acoustic waves

   https://doi.org/10.1039/D1LC00257K  

   Cui, Mingyang; Kim, Minji; Weisensee, Patricia B.; Meacham, J. Mark (June 2021, Lab on a Chip)

   null (Ed.)

   Controlled trapping of cells and microorganisms using substrate acoustic waves (SAWs; conventionally termed surface acoustic waves) has proven useful in numerous biological and biomedical applications owing to the label- and contact-free nature of acoustic confinement. However, excessive heating due to vibration damping and other system losses potentially compromises the biocompatibility of the SAW technique. Herein, we investigate the thermal biocompatibility of polydimethylsiloxane (PDMS)-based SAW and glass-based SAW [that supports a bulk acoustic wave (BAW) in the fluid domain] devices operating at different frequencies and applied voltages. First, we use infrared thermography to produce heat maps of regions of interest (ROI) within the aperture of the SAW transducers for PDMS- and glass-based devices. Motile Chlamydomonas reinhardtii algae cells are then used to test the trapping performance and biocompatibility of these devices. At low input power, the PDMS-based SAW system cannot generate a large enough acoustic trapping force to hold swimming C. reinhardtii cells. At high input power, the temperature of this device rises rapidly, damaging (and possibly killing) the cells. The glass-based SAW/BAW hybrid system, on the other hand, can not only trap swimming C. reinhardtii at low input power, but also exhibits better thermal biocompatibility than the PDMS-based SAW system at high input power. Thus, a glass-based SAW/BAW device creates strong acoustic trapping forces in a biocompatible environment, providing a new solution to safely trap active microswimmers for research involving motile cells and microorganisms. 

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2. Wireless Frequency‐Multiplexed Acoustic Array‐Based Acoustofluidics

   https://doi.org/10.1002/admt.202400572  

   Li, Jiali; Bo, Luyu; Li, Teng; Zhao, Penghui; Du, Yingshan; Cai, Bowen; Shen, Liang; Sun, Wujin; Zhou, Wei; Tian, Zhenhua (July 2024, Advanced Materials Technologies)

   Abstract Acoustofluidics has shown great potential in enabling on‐chip technologies for driving liquid flows and manipulating particles and cells for engineering, chemical, and biomedical applications. To introduce on‐demand liquid sample processing and micro/nano‐object manipulation functions to wearable and embeddable electronics, wireless acoustofluidic chips are highly desired. This paper presents wireless acoustofluidic chips to generate acoustic waves carrying sufficient energy and achieve key acoustofluidic functions, including arranging particles and cells, generating fluid streaming, and enriching in‐droplet particles. To enable these functions, the wireless acoustofluidic chips leverage mechanisms, including inductive coupling‐based wireless power transfer (WPT), frequency multiplexing‐based control of multiple acoustic waves, and the resultant acoustic radiation and drag forces. For validation, the wirelessly generated acoustic waves are measured using laser vibrometry when different materials (e.g., bone, tissue, and hand) are inserted between the WPT transmitter and receiver. Moreover, the wireless acoustofluidic chips successfully arrange nanoparticles into different patterns, align cells into parallel pearl chains, generate streaming, and enrich in‐droplet microparticles. This research is anticipated to facilitate the development of embeddable wireless on‐chip flow generators, wearable sensors with liquid sample processing functions, and implantable devices with flow generation and acoustic stimulation abilities for engineering, veterinary, and biomedical applications. 

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3. Acoustic trap-and-release for rapid assessment of cell motility

   https://doi.org/10.1039/c9sm00184k  

   Kim, Minji; Huff, Emma; Bottier, Mathieu; Dutcher, Susan K.; Bayly, Philip V.; Meacham, J. Mark (May 2019, Soft Matter)

   Functional cilia and flagella are crucial to the propulsion of physiological fluids, motile cells, and microorganisms. Motility assessment of individual cells allows discrimination of normal from dysfunctional behavior, but cell-scale analysis of individual trajectories to represent a population is laborious and impractical for clinical, industrial, and even research applications. We introduce an assay that quantifies swimming capability as a function of the variation in polar moment of inertia of cells released from an acoustic trap. Acoustic confinement eliminates the need to trace discrete trajectories and enables automated analysis of hundreds of cells in minutes. The approach closely approximates the average speed estimated from the mean squared displacement of individual cells for wild-type Chlamydomonas reinhardtii and two mutants ( ida3 and oda5 ) that display aberrant swimming behaviors. Large-population acoustic trap-and-release rapidly differentiates these cell types based on intrinsic motility, which provides a highly sensitive and efficient alternative to conventional particle tracing. 

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4. Cell lysis via acoustically oscillating sharp edges

   https://doi.org/10.1039/C9LC00498J  

   Wang, Zeyu; Huang, Po-Hsun; Chen, Chuyi; Bachman, Hunter; Zhao, Shuaiguo; Yang, Shujie; Huang, Tony J. (December 2019, Lab on a Chip)

   In this article, we demonstrate an acoustofluidic device for cell lysis using the acoustic streaming effects induced by acoustically oscillating sharp-edged structures. The acoustic streaming locally generates high shear forces that can mechanically rupture cell membranes. With the acoustic-streaming-derived shear forces, our acoustofluidic device can perform cell lysis in a continuous, reagent-free manner, with a lysis efficiency of more than 90% over a range of sample flow rates. We demonstrate that our acoustofluidic lysis device works well on both adherent and non-adherent cells. We also validate it using clinically relevant samples such as red blood cells infected with malarial parasites. Additionally, the unique capability of our acoustofluidic device was demonstrated by performing downstream protein analysis and gene profiling without additional washing steps post-lysis. Our device is simple to fabricate and operate while consuming a relatively low volume of samples. These advantages and other features including the reagent-free nature and controllable lysis efficiency make our platform valuable for many biological and biomedical applications, particularly for the development of point-of-care platforms. 

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5. Robust acoustic trapping and perturbation of single-cell microswimmers illuminate three-dimensional swimming and ciliary coordination

   https://doi.org/10.1073/pnas.2218951120  

   Cui, Mingyang; Dutcher, Susan K.; Bayly, Philip V.; Meacham, J. Mark (June 2023, Proceedings of the National Academy of Sciences)

   We report a label-free acoustic microfluidic method to confine single, cilia-driven swimming cells in space without limiting their rotational degrees of freedom. Our platform integrates a surface acoustic wave (SAW) actuator and bulk acoustic wave (BAW) trapping array to enable multiplexed analysis with high spatial resolution and trapping forces that are strong enough to hold individual microswimmers. The hybrid BAW/SAW acoustic tweezers employ high-efficiency mode conversion to achieve submicron image resolution while compensating for parasitic system losses to immersion oil in contact with the microfluidic chip. We use the platform to quantify cilia and cell body motion for wildtype biciliate cells, investigating effects of environmental variables like temperature and viscosity on ciliary beating, synchronization, and three-dimensional helical swimming. We confirm and expand upon the existing understanding of these phenomena, for example determining that increasing viscosity promotes asynchronous beating. Motile cilia are subcellular organelles that propel microorganisms or direct fluid and particulate flow. Thus, cilia are critical to cell survival and human health. The unicellular algaChlamydomonas reinhardtiiis widely used to investigate the mechanisms underlying ciliary beating and coordination. However, freely swimming cells are difficult to image with sufficient resolution to capture cilia motion, necessitating that the cell body be held during experiments. Acoustic confinement is a compelling alternative to use of a micropipette, or to magnetic, electrical, and optical trapping that may modify the cells and affect their behavior. Beyond establishing our approach to studying microswimmers, we demonstrate a unique ability to mechanically perturb cells via rapid acoustic positioning. 

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