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1. I-24 MOTION: An instrument for freeway traffic science

   https://doi.org/10.1016/j.trc.2023.104311  

   Gloudemans, Derek; Wang, Yanbing; Ji, Junyi; Zachár, Gergely; Barbour, William; Hall, Eric; Cebelak, Meredith; Smith, Lee; Work, Daniel B. (October 2023, Transportation Research Part C: Emerging Technologies)
2. Virtual Trajectories for I–24 MOTION: Data and Tools

   https://doi.org/10.1109/FISTS60717.2024.10485593  

   Ji, Junyi; Wang, Yanbing; Gloudemans, Derek; Zachár, Gergely; Barbour, William; Work, Daniel B (February 2024, IEEE)
3. Disruption of a ∼23–24 nucleotide small RNA pathway elevates DNA damage responses in Tetrahymena thermophila

   https://doi.org/10.1091/mbc.E20-10-0631  

   Lee, Suzanne R.; Pollard, Daniel A.; Galati, Domenico F.; Kelly, Megan L.; Miller, Brian; Mong, Christina; Morris, Megan N.; Roberts-Nygren, Kerry; Kapler, Geoffrey M.; Zinkgraf, Matthew; et al (July 2021, Molecular Biology of the Cell)

   Misteli, Tom (Ed.)

   Endogenous RNA interference (RNAi) pathways regulate a wide range of cellular processes in diverse eukaryotes, yet in the ciliated eukaryote, Tetrahymena thermophila, the cellular purpose of RNAi pathways that generate ∼23–24 nucleotide (nt) small (s)RNAs has remained unknown. Here, we investigated the phenotypic and gene expression impacts on vegetatively growing cells when genes involved in ∼23–24 nt sRNA biogenesis are disrupted. We observed slower proliferation and increased expression of genes involved in DNA metabolism and chromosome organization and maintenance in sRNA biogenesis mutants RSP1Δ, RDN2Δ, and RDF2Δ. In addition, RSP1Δ and RDN2Δ cells frequently exhibited enlarged chromatin extrusion bodies, which are nonnuclear, DNA-containing structures that may be akin to mammalian micronuclei. Expression of homologous recombination factor Rad51 was specifically elevated in RSP1Δ and RDN2Δ strains, with Rad51 and double-stranded DNA break marker γ-H2A.X localized to discrete macronuclear foci. In addition, an increase in Rad51 and γ-H2A.X foci was also found in knockouts of TWI8, a macronucleus-localized PIWI protein. Together, our findings suggest that an evolutionarily conserved role for RNAi pathways in maintaining genome integrity may be extended even to the early branching eukaryotic lineage that gave rise to Tetrahymena thermophila. 

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4. Antarctic Isotope Maxima events 24 and 25 identified in benthic marine δ ¹⁸ O: CURRENTS

   https://doi.org/10.1029/2011pa002269  

   Caballero-Gill, Rocio P.; Clemens, Steve; Prell, Warren (March 2012, Paleoceanography)
5. Premeiotic 24-nt phasiRNAs are present in the Zea genus and unique in biogenesis mechanism and molecular function

   https://doi.org/10.1073/pnas.2402285121  

   Zhan, Junpeng; Bélanger, Sébastien; Lewis, Scott; Teng, Chong; McGregor, Madison; Beric, Aleksandra; Schon, Michael A; Nodine, Michael D; Meyers, Blake C (May 2024, Proceedings of the National Academy of Sciences)

   Reproductive phasiRNAs (phased, small interfering RNAs) are broadly present in angiosperms and play crucial roles in sustaining male fertility. While the premeiotic 21-nt (nucleotides) phasiRNAs and meiotic 24-nt phasiRNA pathways have been extensively studied in maize (Zea mays) and rice (Oryza sativa), a third putative category of reproductive phasiRNAs–named premeiotic 24-nt phasiRNAs–have recently been reported in barley (Hordeum vulgare) and wheat (Triticum aestivum). To determine whether premeiotic 24-nt phasiRNAs are also present in maize and related species and begin to characterize their biogenesis and function, we performed a comparative transcriptome and degradome analysis of premeiotic and meiotic anthers from five maize inbred lines and three teosinte species/subspecies. Our data indicate that a substantial subset of the 24-nt phasiRNA loci in maize and teosinte are already highly expressed at the premeiotic phase. The premeiotic 24-nt phasiRNAs are similar to meiotic 24-nt phasiRNAs in genomic origin and dependence on DCL5 (Dicer-like 5) for biogenesis, however, premeiotic 24-nt phasiRNAs are unique in that they are likely i) not triggered by microRNAs, ii) not loaded by AGO18 proteins, and iii) not capable of mediatingPHASprecursor cleavage. In addition, we also observed a group of premeiotic 24-nt phasiRNAs in rice using previously published data. Together, our results indicate that the premeiotic 24-nt phasiRNAs constitute a unique class of reproductive phasiRNAs and are present more broadly in the grass family (Poaceae) than previously known. 

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