Metabolomics aims to achieve a global quantitation of the pool of metabolites within a biological system. Importantly, metabolite concentrations serve as a sensitive marker of both genomic and phenotypic changes in response to both internal and external stimuli. NMR spectroscopy greatly aids in the understanding of both in vitro and in vivo physiological systems and in the identification of diagnostic and therapeutic biomarkers. Accordingly, NMR is widely utilized in metabolomics and fluxomics studies due to its limited requirements for sample preparation and chromatography, its non‐destructive and quantitative nature, its utility in the structural elucidation of unknown compounds, and, importantly, its versatility in the analysis of in vitro, in vivo, and ex vivo samples. This review provides an overview of the strengths and limitations of in vitro and in vivo experiments for translational research and discusses how ex vivo studies may overcome these weaknesses to facilitate the extrapolation of in vitro insights to an in vivo system. The application of NMR‐based metabolomics to ex vivo samples, tissues, and biofluids can provide essential information that is close to a living system (in vivo) with sensitivity and resolution comparable to those of in vitro studies. The success of this extrapolation process is criticallymore »
- Award ID(s):
- 1660921
- Publication Date:
- NSF-PAR ID:
- 10227342
- Journal Name:
- Molecules
- Volume:
- 25
- Issue:
- 21
- Page Range or eLocation-ID:
- 5128
- ISSN:
- 1420-3049
- Sponsoring Org:
- National Science Foundation
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Rapid and automated lipid profiling by nuclear magnetic resonance spectroscopy using neural networks
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- https://doi.org/10.3390/molecules25215128
