Ultrabright fluorescent nanoparticles (NPs) hold great promise for demanding bioimaging applications. Recently, extremely bright molecular crystals of cationic fluorophores were obtained by hierarchical coassembly with cyanostar anion‐receptor complexes. These small‐molecule ionic isolation lattices (SMILES) ensure spatial and electronic isolation to prohibit aggregation quenching of dyes. We report a simple, one‐step supramolecular approach to formulate SMILES materials into NPs. Rhodamine‐based SMILES NPs stabilized by glycol amphiphiles show high fluorescence quantum yield (30 %) and brightness per volume (5000 M−1 cm−1/nm3) with 400 dye molecules packed into 16‐nm particles, corresponding to a particle absorption coefficient of 4×107 M−1 cm−1. UV excitation of the cyanostar component leads to higher brightness (>6000 M−1 cm−1/ nm3) by energy transfer to rhodamine emitters. Coated NPs stain cells and are thus promising for bioimaging.
Ultrabright fluorescent nanoparticles (NPs) hold great promise for demanding bioimaging applications. Recently, extremely bright molecular crystals of cationic fluorophores were obtained by hierarchical coassembly with cyanostar anion‐receptor complexes. These small‐molecule ionic isolation lattices (SMILES) ensure spatial and electronic isolation to prohibit aggregation quenching of dyes. We report a simple, one‐step supramolecular approach to formulate SMILES materials into NPs. Rhodamine‐based SMILES NPs stabilized by glycol amphiphiles show high fluorescence quantum yield (30 %) and brightness per volume (5000 M−1 cm−1/nm3) with 400 dye molecules packed into 16‐nm particles, corresponding to a particle absorption coefficient of 4×107 M−1 cm−1. UV excitation of the cyanostar component leads to higher brightness (>6000 M−1 cm−1/ nm3) by energy transfer to rhodamine emitters. Coated NPs stain cells and are thus promising for bioimaging.
more » « less- NSF-PAR ID:
- 10228389
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Angewandte Chemie International Edition
- Volume:
- 60
- Issue:
- 17
- ISSN:
- 1433-7851
- Page Range / eLocation ID:
- p. 9450-9458
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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