Repulsive electrostatic forces between prion‐like proteins are a barrier against aggregation. In neuropharmacology, however, a prion's net charge (
Repulsive electrostatic forces between prion‐like proteins are a barrier against aggregation. In neuropharmacology, however, a prion's net charge (
- NSF-PAR ID:
- 10231517
- Publisher / Repository:
- Wiley Blackwell (John Wiley & Sons)
- Date Published:
- Journal Name:
- Angewandte Chemie
- Volume:
- 133
- Issue:
- 27
- ISSN:
- 0044-8249
- Page Range / eLocation ID:
- p. 15196-15206
- Format(s):
- Medium: X
- Sponsoring Org:
- National Science Foundation
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Basic Protocol 1 : Labeling HAT protein substrates with azide/alkyne‐biotinAlternate Protocol : Labeling protein substrates of HATs with azide/alkyne‐TAMRA for in‐gel visualizationSupport Protocol 1 : Expression and purification of HAT mutantsSupport Protocol 2 : Synthesis of Ac‐CoA surrogatesBasic Protocol 2 : Streptavidin enrichment of biotinylated HAT substratesBasic Protocol 3 : Chemoproteomic identification of HAT substratesBasic Protocol 4 : Validation of specific HAT substrates with western blotting
