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1. Effects of Prairie Degradation and Restoration on Box Turtle Thermal Ecology

   Yerdon, Gabriel L; Royal, Ethan J; Kross, Chelsea S; Willson, John D (March 2023, Journal of herpetology)

   Prairie habitat loss in the United States has led to population declines in many prairie-associated species, including Ornate Box Turtles (Terrapene ornata). Northwest Arkansas is an intergrade zone between the prairie-dwelling T. ornata and the more forestassociated Three-Toed Box Turtle (Terrapene carolina). As such, limited information exists on the potential differences in physiology and thermal ecology between the two box turtle species and how those differences might influence their habitat use. We addressed gaps in our knowledge of the thermal and spatial ecology of T. ornata and T. carolina with a three-part study. First, we compared the thermal profiles of refugia, open, and vegetated microhabitats across degraded prairie, restored prairie, and adjacent forest macrohabitats using operative temperature models and a linear mixed effect model. Second, we measured total evaporative water loss of both species across a range of body sizes. Finally, we fitted a subset of turtles with iButton data loggers and monitored them in the field to examine carapace temperatures and habitat use. Operative temperature models recorded high, largely homogeneous temperatures across microhabitats in degraded prairie and heterogeneous temperatures across restored prairie microhabitats, while forest habitat maintained stable, cool temperatures. Both species exhibited similar evaporative water loss rates; however, T. ornata experienced a broader range of temperatures in the field. Terrapene ornata were exclusively found in prairie habitat, whereas T. carolina was often found in forested habitats and subsurface refugia. Our results demonstrate key differences in box turtle thermal biology and highlight suboptimal thermal characteristics in degraded prairie and forest habitat that should be considered in prairie restoration and management for T. ornata conservation. 

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2. Consistent Nest Site Selection by Turtles across Habitats with Varying Levels of Human Disturbance

   https://doi.org/10.3390/d15020275  

   Folkerts Caldwell, Molly; López-Pérez, Jorge E.; Warner, Daniel A.; Wolak, Matthew E. (February 2023, Diversity)

   Human disturbance impacts the breeding behavior of many species, and it is particularly important to understand how these human-caused changes affect vulnerable taxa, such as turtles. Habitat alteration can change the amount and quality of suitable nesting habitat, while human presence during nesting may influence nesting behavior. Consequently, both habitat alteration and human presence can influence the microhabitat that females choose for nesting. In the summer of 2019, we located emydid turtle nests in east-central Alabama, USA, in areas with varying levels of human disturbance (high, intermediate, low). We aimed to determine whether turtles selected nest sites based on a range of microhabitat variables comparing maternally selected natural nests to randomly chosen artificial nests. We also compared nest site choice across areas with different levels of human disturbance. Natural nests had less variance in canopy openness and average daily mean and minimum temperature than artificial nests, but microhabitat variables were similar across differing levels of disturbance. Additionally, we experimentally quantified nest predation across a natural to human-disturbed gradient. Nest predation rates were higher in areas with low and intermediate levels of disturbance than in areas with high human disturbance. Overall, these results show that turtles are not adjusting their choices of nest microhabitat when faced with anthropogenic change, suggesting that preserving certain natural microhabitat features will be critical for populations in human-disturbed areas. 

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3. Free-Living Aquatic Turtles as Sentinels of Salmonella spp. for Water Bodies

   https://doi.org/10.3389/fvets.2021.674973  

   Hernandez, Sonia M.; Maurer, John J.; Yabsley, Michael J.; Peters, Valerie E.; Presotto, Andrea; Murray, Maureen H.; Curry, Shannon; Sanchez, Susan; Gerner-Smidt, Peter; Hise, Kelley; et al (July 2021, Frontiers in Veterinary Science)

   Reptile-associated human salmonellosis cases have increased recently in the United States. It is not uncommon to find healthy chelonians shedding Salmonella enterica . The rate and frequency of bacterial shedding are not fully understood, and most studies have focused on captive vs. free-living chelonians and often in relation to an outbreak. Their ecology and significance as sentinels are important to understanding Salmonella transmission. In 2012–2013, Salmonella prevalence was determined for free-living aquatic turtles in man-made ponds in Clarke and Oconee Counties, in northern Georgia (USA) and the correlation between species, basking ecology, demographics (age/sex), season, or landcover with prevalence was assessed. The genetic relatedness between turtle and archived, human isolates, as well as, other archived animal and water isolates reported from this study area was examined. Salmonella was isolated from 45 of 194 turtles (23.2%, range 14–100%) across six species. Prevalence was higher in juveniles (36%) than adults (20%), higher in females (33%) than males (18%), and higher in bottom-dwelling species (31%; common and loggerhead musk turtles, common snapping turtles) than basking species (15%; sliders, painted turtles). Salmonella prevalence decreased as forest cover, canopy cover, and distance from roads increased. Prevalence was also higher in low-density, residential areas that have 20–49% impervious surface. A total of 9 different serovars of two subspecies were isolated including 3 S. enterica subsp. arizonae and 44 S. enterica subsp. enterica (two turtles had two serotypes isolated from each). Among the S. enterica serovars, Montevideo ( n = 13) and Rubislaw ( n = 11) were predominant. Salmonella serovars Muenchen, Newport, Mississippi, Inverness, Brazil, and Paratyphi B. var L(+) tartrate positive (Java) were also isolated. Importantly, 85% of the turtle isolates matched pulsed-field gel electrophoresis patterns of human isolates, including those reported from Georgia. Collectively, these results suggest that turtles accumulate Salmonella present in water bodies, and they may be effective sentinels of environmental contamination. Ultimately, the Salmonella prevalence rates in wild aquatic turtles, especially those strains shared with humans, highlight a significant public health concern. 

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4. Incubation temperature and satiety influence general locomotor and exploratory behaviors in the common snapping turtle (Chelydra serpentina)

   Miller, Soleille; Derenne, Adam; Ellis-Felege, Susan; Rhen, Turk (March 2020, Physiology behavior)

   Temperature during embryogenesis determines sex and has been shown to influence other physiological traits in reptiles. The common snapping turtle (Chelydra serpentina) is an ideal model for testing how temperature impacts behavior in species that display temperature-dependent sex determination. Behavioral assays are crucial to understanding how a changing climate may affect whole organism function in the snapping turtle. Currently, there are few behavioral assays for semi-aquatic vertebrates like turtles. In this study, we used digital cameras to record behavior of fed and fasted hatchling turtles from different incubation temperatures in an open field setting for 20 minutes in 2018 and repeated the experiment in 2019. Open fields were circular tanks filled with water to a depth of 3.5 cm. Each field was split into four quadrants and two zones (inner and outer). The amount of time turtles spent actively moving, total distance travelled, and several other measures were collected and summarized automatically from videos with open source image analysis software (ImageJ). Satiety and incubation temperature had significant effects on total distance moved, time spent moving, and time moving in the outer zone. These findings indicate that temperature during embryogenesis has a long-lasting effect on neural mechanisms underlying exploratory or general locomotor behavior in turtles. 

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5. Biophysical drivers for predicting the distribution and abundance of invasive yellow sweet clover in the Northern Great Plains

   Saraf, Sakshi (March 2023, Landscape ecology)

   Jianguo (Ed.)

   Yellow sweetclover (Melilotus officinalis; YSC) is an invasive biennial legume that bloomed across the Northern Great Plains in 2018–2019 in response to above-average precipitation. YSC can increase nitrogen (N) levels and potentially cause substantial changes in the composition of native plant species communities. There is little knowledge of the spatiotemporal variability and conditions causing substantial widespread blooms of YSC across western South Dakota (SD). We aimed to develop a generalized prediction model to predict the relative abundance of YSC in suitable habitats across rangelands of western South Dakota for 2019. Our research questions are: (1) What is the spatial extent of YSC across western South Dakota? (2) Which model can accurately predict the habitat and percent cover of YSC? and (3) What significant biophysical drivers affect its presence across western South Dakota? We trained machine learning models with in situ data (2016–2021), Sentinel 2A-derived surface reflectance and indices (10 m, 20 m) and site-specific variables of climate, topography, and edaphic factors to optimize model performance. We identified moisture proxies (Shortwave Infrared reflectance and variability in Tasseled Cap Wetness) as the important predictors to explain the YSC presence. Land Surface Water Index and variability in summer temperature were the top predictors in explaining the YSC abundance. We demonstrated how machine learning algorithms could help generate valuable information on the spatial distribution of this invasive plant. We delineated major YSC hotspots in Butte, Pennington, and Corson Counties of South Dakota. The floodplains of major rivers, including White and Bad Rivers, and areas around Badlands National Park also showed a higher occurrence probability and cover percentage. These prediction maps could aid land managers in devising management strategies for the regions that are prone to YSC outbreaks. The management workflow can also serve as a prototype for mapping other invasive plant species in similar regions. 

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