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1. Rhizosphere Microbiomes in a Historical Maize-Soybean Rotation System Respond to Host Species and Nitrogen Fertilization at the Genus and Subgenus Levels

   https://doi.org/10.1128/AEM.03132-20  

   Meier, Michael A. ; Lopez-Guerrero, Martha G. ; Guo, Ming ; Schmer, Marty R. ; Herr, Joshua R. ; Schnable, James C. ; Alfano, James R. ; Yang, Jinliang ( May 2021 , Applied and Environmental Microbiology)

   Liu, Shuang-Jiang (Ed.)

   ABSTRACT Root-associated microbes are key players in plant health, disease resistance, and nitrogen (N) use efficiency. It remains largely unclear how the interplay of biological and environmental factors affects rhizobiome dynamics in agricultural systems. In this study, we quantified the composition of rhizosphere and bulk soil microbial communities associated with maize ( Zea mays L.) and soybean ( Glycine max L.) in a long-term crop rotation study under conventional fertilization and low-N regimes. Over two growing seasons, we evaluated the effects of environmental conditions and several treatment factors on the abundance of rhizosphere- and soil-colonizing microbial taxa. Time of sampling, host plant species, and N fertilization had major effects on microbiomes, while no effect of crop rotation was observed. Using variance partitioning as well as 16S sequence information, we further defined a set of 82 microbial genera and functional taxonomic groups at the subgenus level that show distinct responses to treatment factors. We identified taxa that are highly specific to either maize or soybean rhizospheres, as well as taxa that are sensitive to N fertilization in plant rhizospheres and bulk soil. This study provides insights to harness the full potential of soil microbes in maize and soybean agricultural systems through plant breeding and field management. IMPORTANCE Plant roots are colonized by large numbers of microbes, some of which may help the plant acquire nutrients and fight diseases. Our study contributes to a better understanding of root-colonizing microbes in the widespread and economically important maize-soybean crop rotation system. The long-term goal of this research is to optimize crop plant varieties and field management to create the best possible conditions for beneficial plant-microbe interactions to occur. These beneficial microbes may be harnessed to sustainably reduce dependency on pesticides and industrial fertilizer. We identify groups of microbes specific to the maize or to the soybean host and microbes that are sensitive to nitrogen fertilization. These microbes represent candidates that may be influenced through plant breeding or field management, and future research will be directed toward elucidating their roles in plant health and nitrogen usage. 

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2. Utilizing mycorrhizal responses to guide selective breeding for agricultural sustainability

   https://doi.org/10.1002/ppp3.10200  

   Cobb, Adam B. ; Duell, Eric B. ; Haase, Kaitlin B. ; Miller, R. Michael ; Wu, Yanqi Q. ; Wilson, Gail W. T. ( May 2021 , PLANTS, PEOPLE, PLANET)

   Agriculture touches all aspects of society and global environmental health. Dwindling phosphorous reserves are a looming crisis for civilization, and soil erosion typically far outpaces pedogenesis. Improving plant–mycorrhizal symbioses can enhance sustainable agriculture because mycorrhizas typically improve host‐plant nutrition and stabilize soils. Selective breeding of plants that gain greater benefits from mycorrhizas can provide considerable economic and environmental benefits. Our assessments demonstrate switchgrass genetic improvement increased or maintained production of two populations, and low‐input breeding increased mycorrhizal responsiveness, compared to parent lines. Selecting for increased mycorrhizal reliance may be an effective strategy for more sustainable and economical agricultural production.

   Plant–mycorrhizal interactions are not typically assessed in crop breeding programs. Our experiment addresses this by determining host‐plant outcomes of newly developed synthetic (agronomic) populations compared with parent lines, following low‐input selective breeding. Assessing the potential of low‐input breeding to enhance crop mycorrhizal benefits is a critical step toward more sustainable agricultural production.

   We compared four synthetic populations ofPanicum virgatum, from a low‐input biofuel breeding program at Oklahoma State University, to corresponding parent lines. Plants were grown in a greenhouse in native prairie soils that were either steam‐pasteurized (nonmycorrhizal) or non‐steamed (mycorrhizal).

   We assessed shoot and root biomass, shoot P concentration and P content, mycorrhizal growth response (MGR), and mycorrhizal phosphorous response (MPR). Importantly, we provide novel evidence that low‐input selective breeding increased mycorrhizal reliance of switchgrass synthetics compared to parent lines, with implications for global agricultural systems.

   There are substantial opportunities for plant traits associated with increased MGR and MPR to be transferred to a wide array of crops. Our findings indicate low‐input selective breeding can improve MGR and MPR. We propose these traits serve as a useful proxy for host‐plant mycorrhizal reliance, facilitating successful hologenome breeding to reduce fertilizer requirements.

    

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3. The Impacts of Domestication and Breeding on Nitrogen Fixation Symbiosis in Legumes

   https://doi.org/10.3389/fgene.2020.00973  

   Liu, J ; Yu, X ; Qin, Q ; Dinkins, Randy D ; Zhu, H. ( July 2020 , Frontiers in genetics)

   Legumes are the second most important family of crop plants. One defining feature of legumes is their unique ability to establish a nitrogen-fixing root nodule symbiosis with soil bacteria known as rhizobia. Since domestication from their wild relatives, crop legumes have been under intensive breeding to improve yield and other agronomic traits but with little attention paid to the belowground symbiosis traits. Theoretical models predict that domestication and breeding processes, coupled with high‐input agricultural practices, might have reduced the capacity of crop legumes to achieve their full potential of nitrogen fixation symbiosis. Testing this prediction requires characterizing symbiosis traits in wild and breeding populations under both natural and cultivated environments using genetic, genomic, and ecological approaches. However, very few experimental studies have been dedicated to this area of research. Here, we review how legumes regulate their interactions with soil rhizobia and how domestication, breeding and agricultural practices might have affected nodulation capacity, nitrogen fixation efficiency, and the composition and function of rhizobial community. We also provide a perspective on how to improve legume-rhizobial symbiosis in sustainable agricultural systems. 

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4. Intensification of no‐till agricultural systems: An opportunity for carbon sequestration

   https://doi.org/10.1002/saj2.20260  

   Nicoloso, Rodrigo S. ; Rice, Charles W. ( August 2021 , Soil Science Society of America Journal)

   The “4 per 1,000” initiative was launched at the 21st Conference of the Parties (COP21) stimulating a long‐standing debate on the potential of no‐till (NT) to promote soil C sequestration. Previous reviews found little or no soil organic C (SOC) accrual in NT soils as compared with full inversion tillage when soils are sampled deeper than 30 cm. Here, we present the results of a global meta‐analysis of studies assessing SOC and total N (TN) storage and dynamics in NT and tilled soils from the most important agricultural regions of the world. Overall, our results show that NT soils stored 6.7 ± 1.9 Mg C ha^–1and 1.1 ± 0.4 Mg N ha^–1more than tilled soils (0‐to‐100‐cm depth) with an average of 16 yr of NT, in contrast with previous findings. However, C sequestration (+4.7 ± 1.9 Mg C ha^–1in the 0‐to‐60‐cm depth with an average of 11 yr of NT) depended on the association of NT with increased crop frequency and the inclusion of legumes cover crops. Single‐cropping systems lack the necessary C inputs to offset SOC losses in the soil profile (below 30‐cm depth). However, double‐cropping systems decreased soil TN that may constrain future C sequestration. The use of legumes alleviated TN loss and supported soil C sequestration. Briefly, our findings indicate that NT can avoid SOC losses from tilled soils, partially offsetting CO₂emissions from agriculture. Moreover, NT with agricultural intensification can promote soil C sequestration, thus contributing to soil quality, food security, and adaptation to climate change.

    

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5. Uncertainties in estimating global potential yields and their impacts for long-term modeling

   https://doi.org/10.1007/s12571-021-01228-x  

   Ollenburger, Mary ; Kyle, Page ; Zhang, Xin ( March 2022 , Food Security)

   Estimating realistic potential yields by crop type and region is challenging; such yields depend on both biophysical characteristics (e.g., soil characteristics, climate, etc.), and the crop management practices available in any site or region (e.g., mechanization, irrigation, crop cultivars). A broad body of literature has assessed potential yields for selected crops and regions, using several strategies. In this study we first analyze future potential yields of major crop types globally by two different estimation methods, one of which is based on historical observed yields (“Empirical”), while the other is based on biophysical conditions (“Simulated”). Potential yields by major crop and region are quite different between the two methods; in particular, Simulated potential yields are typically 200% higher than Empirical potential yields in tropical regions for major crops. Applying both of these potential yields in yield gap closure scenarios in a global agro-economic model, GCAM, the two estimates of future potential yields lead to very different outcomes for the agricultural sector globally. In the Simulated potential yield closure scenario, Africa, Asia, and South America see comparatively favorable outcomes for agricultural sustainability over time: low land use change emissions, low crop prices, and high levels of self-sufficiency. In contrast, the Empirical potential yield scenario is characterized by a heavy reliance on production and exports in temperate regions that currently practice industrial agriculture. At the global level, this scenario has comparatively high crop commodity prices, and more land allocated to crop production (and associated land use change emissions) than either the baseline or Simulated potential yield scenarios. This study highlights the importance of the choice of methods of estimating potential yields for agro-economic modeling.

    

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