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Title: Hyperspectral Three-Dimensional Fluorescence Imaging Using Snapshot Optical Tomography
Hyperspectral three-dimensional (3D) imaging can provide both 3D structural and functional information of a specimen. The imaging throughput is typically very low due to the requirement of scanning mechanisms for different depths and wavelengths. Here we demonstrate hyperspectral 3D imaging using Snapshot projection optical tomography (SPOT) and Fourier-transform spectroscopy (FTS). SPOT allows us to instantaneously acquire the projection images corresponding to different viewing angles, while FTS allows us to perform hyperspectral imaging at high spectral resolution. Using fluorescent beads and sunflower pollens, we demonstrate the imaging performance of the developed system.  more » « less
Award ID(s):
1808331
NSF-PAR ID:
10274176
Author(s) / Creator(s):
; ;
Date Published:
Journal Name:
Sensors
Volume:
21
Issue:
11
ISSN:
1424-8220
Page Range / eLocation ID:
3652
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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