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Title: Methods to Investigate the Kinetic Profile of Cysteine Desulfurases
Biological iron-sulfur (Fe-S) clusters are essential protein prosthetic groups that promote a range of biochemical reactions. In vivo, these clusters are synthesized by specialized protein machineries involved in sulfur mobilization, cluster assembly, and cluster transfer to their target proteins. Cysteine desulfurases initiate the first step of sulfur activation and mobilization in cluster biosynthetic pathways. The reaction catalyzed by these enzymes involves the abstraction of sulfur from the amino acid l-cysteine, with concomitant formation of alanine. The presence and availability of a sulfur acceptor modulate the sulfurtransferase activity of this class of enzymes by altering their reaction profile and catalytic turnover rate. Herein, we describe two methods used to probe the reaction profile of cysteine desulfurases through quantification of alanine and sulfide production in these reactions.  more » « less
Award ID(s):
1716535
NSF-PAR ID:
10287907
Author(s) / Creator(s):
; ;
Editor(s):
Dos Santos, P.C.
Date Published:
Journal Name:
Methods in molecular biology
Volume:
2353
ISSN:
1064-3745
Page Range / eLocation ID:
173-189
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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