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Title: Depth-of-field extension in optical imaging for rapid crystal screening
The depth of field (DoF) was extended 2.8-fold to achieve rapid crystal screening by retrofitting a custom-designed micro-retarder array (µRA) in the optical beam path of a nonlinear optical microscope. The merits of the proposed strategy for DoF enhancement were assessed in applications of second-harmonic generation imaging of protein crystals. It was found that DoF extension increased the number of crystals detected while simultaneously reducing the number of ` z -slices' required for screening. Experimental measurements of the wavelength-dependence of the extended DoF were in excellent agreement with theoretical predictions. These results provide a simple and broadly applicable approach to increase the throughput of existing nonlinear optical imaging methods for protein crystal screening.  more » « less
Award ID(s):
2004046
PAR ID:
10293979
Author(s) / Creator(s):
; ; ; ; ;
Date Published:
Journal Name:
Acta Crystallographica Section D Structural Biology
Volume:
77
Issue:
4
ISSN:
2059-7983
Page Range / eLocation ID:
463 to 470
Format(s):
Medium: X
Sponsoring Org:
National Science Foundation
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